Two Stages of N-Deficient Cultivation Enhance the Lipid Content of Microalga Scenedesmus sp.

详细信息    查看全文

• 作者：Xiaomei Li ; Mingzi Wang ; Xinhui Liao…
• 关键词：Scenedesmus sp. ; Two ; stage N ; deficient cultivation ; Nitrogen deficiency ; Lipid
• 刊名：Journal of the American Oil Chemists' Society
• 出版年：2015
• 出版时间：April 2015
• 年：2015
• 卷：92
• 期：4
• 页码：503-512
• 全文大小：489 KB
• 参考文献：1. Prabakaran, P, Ravindran, A (2012) Scenedesmus as a potential source of biodiesel among selected microalgae. Curr Sci 102: pp. 616-620
  2. Weyer, KM, Bush, DR, Darzins, A, Willson, BD (2010) Theoretical maximum algal oil production. Bioenerg Res 3: pp. 204-213 CrossRef
  3. Mutanda, T, Ramesh, D, Karthikeyan, S, Kumari, S, Anandraj, A, Bux, F (2011) Bioprospecting for hyper-lipid producing microalgal strains for sustainable biofuel production. Bioresour Technol 102: pp. 57-70 CrossRef
  4. Chen, Z, Gong, Y, Fang, X, Hu, H (2012) Scenedesmus sp. NJ-1 isolated from Antarctica: a suitable renewable lipid source for biodiesel production. World J Microb Biot 28: pp. 3219-3225 CrossRef
  5. Mandal, S, Mallick, N (2011) Waste utilization and biodiesel production by the green microalga Scenedesmus obliquus. Appl Environ Microb 77: pp. 374-377 CrossRef
  6. de Queiroz JC, de Melo Ferreira AC, da Costa ACA (2012) The growth of / Monoraphidium sp. and / Scenedesmus sp. cells in the presence of thorium. Sci World J 2012:1-. Art ID 592721
  7. Yeesang, C, Cheirsilp, B (2011) Effect of nitrogen, salt, and iron content in the growth medium and light intensity on lipid production by microalgae isolated from freshwater sources in Thailand. Bioresour Technol 102: pp. 3034-3040 CrossRef
  8. Chu, FF, Chu, PN, Cai, PJ, Li, WW, Lam, PKS, Zeng, RJ (2013) Phosphorus plays an important role in enhancing biodiesel productivity of Chlorella vulgaris under nitrogen deficiency. Bioresour Technol 134: pp. 341-346 CrossRef
  9. Pokora, W, Aksmann, A, Tukaj, Z (2011) Functional characteristics of green alga Scenedesmus obliquus (Chlorophyceae): 276-6 wild type and its two photosystems deficient mutants cultured under photoautotrophic, mixotrophic and heterotrophic conditions. Phycol Res 59: pp. 259-268 CrossRef
  10. Jin, H-F, Lim, B-R, Lee, K (2006) Influence of nitrate feeding on carbon dioxide fixation by microalgae. J Environ Sci Heal A 41: pp. 2813-2824 CrossRef
  11. Feng, D, Chen, Z, Xue, S, Zhang, W (2011) Increased lipid production of the marine oleaginous microalga Isochrysis zhangjiangensis (Chrysophyta) by nitrogen supplement. Bioresour Technol 102: pp. 6710-6716 CrossRef
  12. Li, X, Liao, X, Chen, B, Wang, (2014) Improvement of biomass and lipid content of Scenedesmus sp. by two-step cultivation. China Oil Fat 39: pp. 53-56
  13. Sun, X, Cao, Y, Xu, H, Liu, Y, Sun, JR, Qiao, DR, Cao, Y (2014) Effect of nitrogen-starvation, light intensity and iron on triacylglyceride/carbohydrate production and fatty acid profile of Neochloris oleoabundans HK-129 by a two-stage process. Bioresour Technol 155: pp. 204-212 CrossRef
  14. Mujtaba, G, Choi, W, Lee, C-G, Lee, K (2012) Lipid production by Chlorella vulgaris after a shift from nutrient-rich to nitrogen starvation conditions. Bioresour Technol 123: pp. 279-283 CrossRef
  15. Liu, J, Yuan, C, Hu, G, Li, F (2012) Effects of light intensity on the growth and lipid accumulation of microalga Scenedesmus sp. 11- under nitrogen limitation. Appl Biochem Biotechnol 166: pp. 2127-2137 CrossRef
  16. Li, Y, Horsman, M, Wang, B, Wu, N, Lan, CQ (2008) Effects of nitrogen sources on cell growth and lipid accumulation of green alga Neochloris oleoabundans. Appl Microbiol Biot 81: pp. 629-636 CrossRef
  17. Beschkov, V, Velizarov, S, Agathos, S, Lukova, V (2
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Industrial Chemistry and Chemical Engineering
  Analytical Chemistry
  Chemistry
  Biotechnology
  Biomaterials
  Agriculture
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1558-9331

文摘

Scenedesmus sp. 14-3 was identified as a suitable candidate for producing biodiesel. The present work studied the effects of nitrogen concentration on the biomass and lipid productivity of algae, the consumption of sodium nitrate, and the two-stage N-deficient cultivation that could enhance dramatically the accumulation of biomass and lipids of Scenedesmus sp. 14-3. The two-stage N-deficient cultivation was described as follows: microalga Scenedesmus sp. 14-3 was cultured under low light intensity (LL) for 10?days in an N-deficient medium by 20?% inoculum concentration, and transferred to complete N-depletion BG11 under high light intensity (HL) for 8?days. The highest lipid content of Scenedesmus sp. 14-3 was 53.05?±?0.08?% (10?% inoculum concentration) following the second stage of N-deficient cultivation after 8?days. For the second stage of N-deficient cultivation, the lipid content of Scenedesmus sp. 14-3 was 49.85?±?0.22?%, which was 1.8 times higher than that under low light intensity (LL) (46-8?μmol?m??s? ) in 10?days. Meanwhile, the high algal biomass productivity was around 0.10?g L? day? after the first stage of N-deficient cultivation (10?days) and the biomass productivity was around 0.037?g?L??day? under the second stage of N-deficient cultivation (8?days). The comparison under different culture conditions showed a significant effect of the two-stage of N-deficient cultivation on lipid accumulation of Scenedesmus sp. 14-3. The two-stage N-deficient cultivation without centrifugation achieved a complete N-depletion condition, but the two-stage process required centrifugation which is unsuitable for commercialization and large-scale utilization. In summary, two-stage N-deficient cultivation is a more suitable and effective culture method for commercial applications and dramatic accumulation of lipids than the two-stage process.