A “GC-rich-method for mammalian gene expression: A dominant role of non-coding DNA GC content in regulation of mammalian gene expression
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- 作者：Qian Jia (1)
HongTao Wu (1)
XingJun Zhou (1)
Jian Gao (1)
Wei Zhao (1)
JouDi Aziz (2) (4)
JingShuang Wei (1)
Lihua Hou (5)
Shuyin Wu (4)
Ying Zhang (3)
XiangFeng Dong (1)
YanMin Huang (1)
WeiYuan Jin (4)
HongJie Zhu (3)
XinHui Zhao (5)
ChunHua Huang (6)
LiPing Xing (4)
Liwen Li (4)
Jun Ma (4)
Xiyan Liu (4)
Ran Tao (4)
ShuaiDong Ye (4)
YiGao Song (4)
LingLing Song (4)
GuanPing Chen (4)
ChunLing Du (4)
XueTing Zhang (3)
Bo Li (3)
YanTao Wang (3)
Wei Yang (3)
Gilbert Rishton (2)
YuYang Teng (3)
GouQing Leng (3)
LuanFeng Li (4)
WenXian Liu (1)
LiJun Cheng (1)
QiuBo Liang (3)
ZhengWu Li (3)
XiuQin Zhang (3)
Yajun Zuo (6)
Wei Chen (5)
Huicheng Li (3)
Matthew (Mizhou) Hui (1) (2) (3) (4) - 关键词：GC ; rich ; GC content ; DNA structure ; stable expression ; mammalian cell ; expression mechanism
- 刊名：Science China Life Sciences
- 出版年：2010
- 出版时间：January 2010
- 年：2010
- 卷：53
- 期：1
- 页码：94-100
- 全文大小：1931KB
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HongTao Wu (1)
XingJun Zhou (1)
Jian Gao (1)
Wei Zhao (1)
JouDi Aziz (2) (4)
JingShuang Wei (1)
Lihua Hou (5)
Shuyin Wu (4)
Ying Zhang (3)
XiangFeng Dong (1)
YanMin Huang (1)
WeiYuan Jin (4)
HongJie Zhu (3)
XinHui Zhao (5)
ChunHua Huang (6)
LiPing Xing (4)
Liwen Li (4)
Jun Ma (4)
Xiyan Liu (4)
Ran Tao (4)
ShuaiDong Ye (4)
YiGao Song (4)
LingLing Song (4)
GuanPing Chen (4)
ChunLing Du (4)
XueTing Zhang (3)
Bo Li (3)
YanTao Wang (3)
Wei Yang (3)
Gilbert Rishton (2)
YuYang Teng (3)
GouQing Leng (3)
LuanFeng Li (4)
WenXian Liu (1)
LiJun Cheng (1)
QiuBo Liang (3)
ZhengWu Li (3)
XiuQin Zhang (3)
Yajun Zuo (6)
Wei Chen (5)
Huicheng Li (3)
Matthew (Mizhou) Hui (1) (2) (3) (4)
1. NCPC R&D Center, Shijiazhuang, 050015, China
2. AmProtein Inc, Camarillo, CA, 93010, USA
4. AmProtein China, Hangzhou, 310019, China
5. Institute of Microbioloy and Epidemiology Academy of Military Medical Sciences, Beijing, 100071, China
3. Harbin Pharmaceutical Group R&D Center, Harhin, 150086, China
6. Shanghai Huayi Biotech, Shanghai, 200233, China
文摘
High mammalian gene expression was obtained for more than twenty different proteins in different cell types by just a few laboratory scale stable gene transfections for each protein. The stable expression vectors were constructed by inserting a naturally-occurring 1.006 kb or a synthetic 0.733 kb DNA fragment (including intron) of extremely GC-rich at the 5-or/and 3-flanking regions of these protein genes or their gene promoters. This experiment is the first experimental evidence showing that a non-coding extremely GC-rich DNA fragment is a super “chromatin opening element-and plays an important role in mammalian gene expression. This experiment has further indicated that chromatin-based regulation of mammalian gene expression is at least partially embedded in DNA primary structure, namely DNA GC-content.