Peroxisome proliferator activated receptor- ligands induced cell growth inhibition and its influence on m

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• 作者：Jiajun Liu ; Huiling Lu ; Renwei Huang ; Dongjun Lin ; Xiangyuan Wu ; Qu Lin ; Xinyao Wu ; Jing Zheng ; Xianglin Pan and Jun Peng ; et al.
• 关键词：MMP ; PPAR ; Leukemia ; ECM
• 刊名：Cancer Chemotherapy and Pharmacology
• 出版年：2005
• 出版时间：October 2005
• 年：2005
• 卷：56
• 期：4
• 页码：400-408
• 全文大小：522 KB

文摘

Peroxisome proliferator-activated receptor-gamma (PPAR-) is one of the best characterized nuclear hormone receptors (NHRs) in the superfamily of ligand-activated transcriptional factors. PPAR- ligands have recently been demonstrated to affect proliferation, differentiation and apoptosis of different cell types. The present study was undertaken to investigate PPAR- ligands induced cell growth inhibition and its influence on matrix metalloproteinase MMP-9 and MMP-2 activities on leukemia K562 and HL-60 cells in vitro. The results revealed that PPAR- expression was detectable in the two kinds of leukemia cells; Both 15-deoxy-delta(12,14)-prostaglandin J2(15d-PGJ2) and troglitazone (TGZ) have significant growth inhibition effects on these two kinds of leukemia cells. These two PPAR- ligands could inhibit the leukemic cell adhesion to the extracellular matrix (ECM) proteins and the invasion through matrigel matrix. The expressions of MMP-9 and MMP-2 as well as their gelatinolytic activities in both HL-60 and K562 cells were inhibited by 15d-PGJ2 and TGZ significantly. We therefore conclude that PPAR- ligands 15d-PGJ2 and TGZ have significant growth inhibition effects on myeloid leukemia cells in vitro, and that PPAR- ligands can inhibit K562 and HL-60 cell adhesion to and invasion through ECM as well as downregulate MMP-9 and MMP-2 expressions. The data suggest that PPAR- ligands may serve as potential anti-leukemia reagents.