Construction of a camelid VHH yeast two-hybrid library and the selection of VHH against haemagglutinin-neuraminidase protein of the Newcastle disease virus

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• 作者：Xiaolong Gao ; Xiangyun Hu ; Lina Tong ; Dandan Liu ; Xudong Chang…
• 关键词：Newcastle disease virus ; VHH ; HN protein ; Yeast two ; hybrid
• 刊名：BMC Veterinary Research
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：12
• 期：1
• 全文大小：1,924 KB
• 刊物主题：Veterinary Medicine; Zoology; Transgenics;
• 出版者：BioMed Central
• ISSN：1746-6148

文摘

Background Newcastle disease (ND), which is caused by the Newcastle disease virus (NDV), is one of the most important avian diseases in poultry. Since its discovery in 1926, ND has caused great economic losses to the world poultry industry and remains a threat to chickens and wild birds. Although a stringent vaccination policy is widely adopted to control ND, ND outbreaks still occur, and virulent NDV is sporadically isolated from chickens and wild birds. To study the pathogenesis of ND and provide tools to prevent its prevalence, novel antibody fragments should be developed. The variable domains of the heavy chain of the heavy-chain antibodies (VHH) are the smallest naturally occurring antibodies derived from camelid heavy-chain antibodies. The comparatively small size, high affinity, high solubility, low immunogenicity and ability to bind epitopes inaccessible to conventional antibodies of VHH make them ideal candidates for a considerable number of therapeutic and biotechnological applications. However, an anti-NDV VHH has not been reported to date.