A parallel and quantitative cell migration assay using a novel multi-well-based device
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- 作者：Qianghua Quan ; Shuwen Zhang ; Xudong Wang ; Qi Ouyang ; Yugang Wang…
- 关键词：Migration assay ; Cancer cells ; Scratch test ; High ; throughput screening
- 刊名：Biomedical Microdevices
- 出版年：2016
- 出版时间：December 2016
- 年：2016
- 卷：18
- 期：6
- 全文大小：5,294 KB
- 刊物类别：Engineering
- 刊物主题：Biomedical Engineering
Biophysics and Biomedical Physics
Nanotechnology
Engineering Fluid Dynamics - 出版者：Springer Netherlands
- ISSN：1572-8781
- 卷排序：18
文摘
Cell migration assays for different chemical environments are important for both scientists and clinicians searching for new therapeutics. In this study, we developed a multi-well-based microfluidic chip that has multiple units for different conditions. In each unit, cells can be patterned and then released to observe their migration. Automatic image analysis and model-based data processing were developed to describe the integrated cell migration assay precisely and quickly. As a demonstration, the migration behaviors of two types of cells in eight chemical conditions were studied. The results showed that supplementation with transforming growth factor-β(TGF-β) significantly promoted the migration of MCF-7 and MCF-10 A cells compared to several growth factors, such as Epidermal Growth Factor(EGF) and basic fibroblast growth factor(bFGF), as well as a control sample. Cells can migrate particularly fast with two or more mixed supplementary factors, such as TGF-β + bFGF + EGF, which indicated a synergy effect. Thus, this chip could be used to quantitatively observe cancer cell migration and demonstrated great potential for use in quantitative migration studies and chemical screening.