Effect of puerarin on transcriptome of astrocyte during oxygen-glucose deprivation/reoxygenation injury
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- 作者：Shuyong Wei ; Jie Tong ; Qiang Xue ; Yang Liu…
- 关键词：Puerarin ; Astrocytes ; OGD/R ; Transcriptome ; IL ; 6/JAK2/STAT3 pathway
- 刊名：Molecular and Cellular Biochemistry
- 出版年：2017
- 出版时间：January 2017
- 年：2017
- 卷：425
- 期：1-2
- 页码：113-123
- 全文大小：
- 刊物类别：Biomedical and Life Sciences
- 刊物主题：Biochemistry, general; Medical Biochemistry; Oncology; Cardiology;
- 出版者：Springer US
- ISSN：1573-4919
- 卷排序：425
文摘
Stroke is a serious disease with complex pathomechanism and limited therapeutic effect in clinic. Our previous research has found obvious therapeutic effect of Puerarin (Pur) on stroke injury of rat. The aim of this study is to investigate the transcriptome changes of oxygen-glucose deprivation/reoxygenation (OGD/R)-injured astrocytes before and after the intervention of Pur. Cells activity and apoptosis detection indicated that the activity of OGD/R-injured astrocytes was improved, and the apoptosis was ameliorated by Pur. Affymetrix GeneChip Rat Genome 230 2.0 Array assays indicated that after intervention of Pur, mRNA expressions of 31 genes were up-regulated and 40 genes were down-regulated in OGD group, whereas mRNA expression of 36 genes were up-regulated, and 88 genes were down-regulated in OGD/R group. Pathway analysis indicated that the olfactory transduction pathway and the JAK (janus kinase) 2/STAT (signal transducer and activator of transcription) three pathways were down-regulated by Pur during OGD/R injury of astrocytes. These data indicated that Pur regulates transcriptome and expresses protective effect on astrocytes during OGD/R injury, and may be a potential therapeutic agent for the treatment of stroke.