Molecular diversity of microbial community in acid mine drainages of Yunfu sulfide mine

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• 作者：Zhiguo He ; Shengmu Xiao ; Xuehui Xie ; Hui Zhong ; Yuehua Hu ; Qinghua Li ; Fenglin Gao ; Guiyuan Li ; Jianshe Liu and Guanzhou Qiu
• 关键词：Microbial diversity ; Ecology ; RFLP ; AMD
• 刊名：Extremophiles
• 出版年：2007
• 出版时间：March, 2007
• 年：2007
• 卷：11
• 期：2
• 页码：305-314
• 全文大小：464 KB
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Microbiology
  Biotechnology
  Ecology
  
• 出版者：Springer Japan
• ISSN：1433-4909

文摘

Two acid mine drainage (AMD) samples were studied by a PCR-based cloning approach, which were from Yunfu sulfide mine in Guangdong province, China. A total of 15 operational taxonomic units (OTUs) were obtained from the two AMD samples. The percentage of overlapped OTUs in two AMD samples was 42.1%. Phylogenetic analysis revealed that the bacterium in the two samples fell into four putative divisions, which were Nitrospira, α-Proteobacteria, β-Proteobacteria, and γ-Proteobacteria four families. Organisms of genuses Acidithiobacillus and Gallionella, which were in γ-Proteobacteria family and β-Proteobacteria family, respectively, were dominant in two samples. The proportions of clones affiliated with Gallionella in each sample were 47.2% (G2) and 16.9% (G1). The result suggested that organisms of Gallionella were a very important composition in microbial communities of the two AMD samples we studied. In addition, the PCR amplification of archaeal 16S rDNA genes form these two AMD samples have been performed with two sets of archaea-specific primers, but no PCR product found.