Somatic embryogenesis from mature zygotic embryos of Distylium chinense (Fr.) Diels and assessment of genetic fidelity of regenerated plants by SRAP markers
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- 作者：Xiaoling Li (1)
Xuesong Wang (1)
Chunyan Luan (1)
Jin Yang (1)
Suihan Cheng (1)
Zelong Dai (1)
Pengsen Mei (1)
Chengming Huang (2) - 关键词：Distylium chinense ; Somatic embryogenesis ; Plant regeneration ; Mature zygotic embryos ; Genetic stability ; SRAP
- 刊名：Plant Growth Regulation
- 出版年：2014
- 出版时间：September 2014
- 年：2014
- 卷：74
- 期：1
- 页码：11-21
- 全文大小：714 KB
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Xuesong Wang (1)
Chunyan Luan (1)
Jin Yang (1)
Suihan Cheng (1)
Zelong Dai (1)
Pengsen Mei (1)
Chengming Huang (2)
1. Engineering Research Center of Eco-Environment in Three Gorges Reservoir Region, Ministry of Education, China Three Gorges University, Yichang, 443002, China
2. Three Gorges Botanical Garden, Yichang, 443111, Hubei, China - ISSN：1573-5087
文摘
The objective was to establish an efficient regeneration protocol for Distylium chinense based on somatic embryogenesis and evaluate the genetic stability of plants regenerated in vitro. To induce callus mature zygotic embryos were cultured on Murashige and Skoog’s (MS) medium that was supplemented with different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) and N6-benzyladenine (BA). After 20?days, the highest rate of callus formation (88.9?%) occurred on MS medium supplemented with 0.5?mg?l? 2,4-D and 0.1?mg?l? BA. It was observed that light-yellow, compact, dry, nodular embryogenic calli had formed. These calli were then subcultured on fresh MS medium supplemented with 0.1?mg?l? BA and 0.5?mg?l? α-naphthaleneacetic acid (NAA) for proliferation for an additional 30?days. To induce somatic embryos and plant regeneration, the embryogenic callus was transferred to fresh MS medium that was supplemented with different concentrations of BA and NAA. After 30?days, 0.5?mg?l? BA in combination with 0.5?mg?l? NAA produced the best result in terms of somatic embryogenesis (%), shoot differentiation (%), number of shoots per callus and shoot length. Next, the plantlets were transferred to the field for 5?weeks and a 95?% survival rate was observed. The sequence-related amplified polymorphism markers confirmed genetic stability of plants regenerated in vitro. To our knowledge, this is the first report that describes a plant regeneration protocol for D. chinense via somatic embryogenesis to be used for germplasm conservation and commercial cultivation.