Characterization, modification, and overexpression of 3-phosphoglycerate dehydrogenase in Corynebacterium glutamicum for enhancing l-serine production

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• 作者：Guoqiang Xu ; Xuexia Jin ; Wen Guo ; Wenfang Dou ; Xiaomei Zhang…
• 关键词：l ; Serine ; Corynebacterium glutamicum ; PGDH ; Feedback inhibition ; Fermentation
• 刊名：Annals of Microbiology
• 出版年：2015
• 出版时间：June 2015
• 年：2015
• 卷：65
• 期：2
• 页码：929-935
• 全文大小：503 KB
• 参考文献：Eggeling L, Bott M (eds) (2005) Handbook of Corynebacterium glutamicum. CRC, Boca Raton
  Eggeling L, Sahm H (1999) l -Glutamate and l -lysine: traditional products with impetuous developments. Appl Microbiol Biotechnol 52:146-53View Article
  Ema M, Kakimoto T, Chibata I (1979) Production of l -serine by Sarcina albida. Appl Environ Microbiol 37:1053-058PubMed Central PubMed
  Grant GA (1989) A new family of 2-hydroxyacid dehydrogenases. Biochem Biophys Res Commun 165:1371-374View Article PubMed
  Grant GA, Schuller DJ, Banaszak LJ (1996) A model for the regulation of d -3-phosphoglycerate dehydrogenase, a Vmax-type allosteric enzyme. Protein Sci 5:34-1View Article PubMed Central PubMed
  Hagishita T, Yoshida T, Izumi Y, Mitsunaga T (1996) Efficient L-serine production from methanol and glycine by resting cells of Methylobacterium sp. strain MN43. Biosci Biotechnol Biochem 60:1604-607View Article PubMed
  Ho CL, Noji M, Saito M, Saito K (1999) Regulation of serine biosynthesis in Arabidopsis. Crucial role of plastidic 3-phosphoglycerate dehydrogenase in non-photosynthetic tissues. J Biol Chem 274:397-02View Article PubMed
  Hsiao HY, Wei T (1986) Enzymatic production of l -serine with a feedback control system for formaldehyde addition. Biotechnol Bioeng 28:1510-518View Article PubMed
  Izumi Y, Yoshida T, Miyazaki SS, Mitsunaga T, Ohshiro T, Shimao M, Miyata A, Tanabe T (1993) l -Serine production by a methylotroph and its related enzymes. Appl Microbiol Biotechnol 39:427-32View Article PubMed
  Lai SJ, Zhang Y, Liu SW, Liang Y, Shang XL, Chai X, Wen TY (2012) Metabolic engineering and flux analysis of Corynebacterium glutamicum for l -serine production. Sci China Life Sci 55:283-90View Article PubMed
  Li Y, Chen GK, Tong XW, Zhang HT, Liu XG, Liu YH, Lu FP (2012) Construction of Escherichia coli strains producing l -serine from glucose. Biotechnol Lett 34:1525-530View Article PubMed
  Marx A, de Graaf AA, Wiechert W, Eggeling L, Sahm H (1996) Determination of the fluxes in the central metabolism of Corynebacterium glutamicum by nuclear magnetic resonance spectroscopy combined with metabolite balancing. Biotechnol Bioeng 49:111-29View Article PubMed
  Netzer R, Peters-Wendisch P, Eggeling L, Sahm H (2004) Cometabolism of a nongrowth substrate: l -serine utilization by Corynebacterium glutamicum. Appl Environ Microb 70:7148-155View Article
  Omori K, Kakimoto T, Chibata I (1983) l -Serine production by a mutant of Sarcina albida defective in l -serine degradation. Appl Environ Microbiol 45:1722-726PubMed Central PubMed
  Peters-Wendisch P, Netzer R, Eggeling L, Sahm H (2002) 3-phosphoglycerate dehydrogenase from Corynebacterium glutamicum: the C-terminal domain is not essential for activity but is required for inhibition by l -serine. Appl Microbiol Biotechnol 60:437-41View Article PubMed
  Peters-Wendisch P, Stolz M, Etterich H, Kennerknecht N, Sahm H, Eggeling L (2005) Metabolic engineering of Corynebacterium glutamicum for l -serine production. Appl Environ Microbiol 71:7139-144View Article PubMed Central PubMed
  Saski R, Pizer LI (1975) Regulatory properties of purified 3-phosphoglycerate dehydrogenase from Bacillus subtilis. Eur J Biochem 51:415-27View Article PubMed
  Sauer U, Eikmanns BJ (2005) The PEP-pyruvate-oxaloacetate node as the switch point for carbon flux distribution in bacteria. FEMS Microbiol Rev 29:765-94View Article PubMed
  Schuller DJ, Grant GA, Banaszak LJ (1995) The allosteric ligand site in the Vmax-type cooperative enzyme phosphoglycerate dehydrogenase. Nat Struct Biol 2:69-6View Article PubMed
  Schweitzer JE, Stolz M, Diesveld R, Etterich H, Eggeling L (2009) The serine hydroxymethyltransferase gene glyA in Corynebacterium glutamicum is controlled by GlyR. J Biotechnol 139:214-21
  Stauffer GV (1996) Biosynthesis of serine, glycine, and one-carbon units, 2nd edn. ASM, Washington, DC
  Vallino JJ, Stephanopoulos G (1994) Carbon flux distributions at the pyruvate branch point in Corynebacterium glutamicum during lysine overproduction. Biotechnol Prog 10:320-26View Article
  Zhang X, Dou W, Xu H, Xu Z (2010) Metabolic flux analysis of l -serine synthesis by Corynebacterium glutamicum SYPS-062. Chinese J Biotechol 26:1363-371
  Zhao G, Winkler ME (1996) A novel alpha-ketoglutarate reductase activity of the serA-encoded 3-phosphoglycerate dehydrogenase of Escherichia coli K-12 and its possible implications for human 2-hydroxyglutaric aciduria. J Bacteriol 178:232-39PubMed Central PubMed
  
• 作者单位：Guoqiang Xu (1) (2) (3)
  Xuexia Jin (1) (3)
  Wen Guo (1) (3)
  Wenfang Dou (1) (3)
  Xiaomei Zhang (1) (2) (3)
  Zhenghong Xu (1) (2) (3)
  
  1. Laboratory of Pharmaceutical Engineering, School of Pharmaceutics Science, Jiangnan University, Wuxi, 214122, China
  2. National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi, 214122, China
  3. The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology Jiangnan University, Wuxi, 214122, China
  
• 刊物主题：Microbiology; Microbial Genetics and Genomics; Microbial Ecology; Fungus Genetics; Medical Microbiology; Applied Microbiology;
• 出版者：Springer Berlin Heidelberg
• ISSN：1869-2044

文摘

The direct fermentative production of l-serine from renewable biomass using Corynebacterium glutamicum is attracting increasing attention. In this study, wild-type C. glutamicum SYPS-062 produced up to 6.65?±-.23?g/L l-serine; to further improve l-serine production, the serA gene was cloned, and the C-terminal domain of 3-phosphoglycerate dehydrogenase (PGDH) from this strain was truncated. When expressed in Escherichia coli, the resultant mutein SerAΔ197 showed a specific PGDH activity of 1.092?±-.05 U/mg protein, representing a decrease of 25.87?% from that encoded by serA, and was no longer sensitive to high concentrations of l-serine. When serA Δ591 was overexpressed in C. glutamicum SYPS-062, the activity of PGDH in C. glutamicum pJC1-tac-serA Δ591 increased by 47.72?%, and the resultant strain C. glutamicum pJC1-tac-serA Δ591 could accumulate 7.69?±-.22?g/L l-serine. Furthermore, when serA Δ591 was overexpressed in C. glutamicum SYPS-062ΔsdaA, the resultant strain could accumulate 8.84?±-.23?g/L l-serine at 102?h, and the yield of l-serine on cells (Y p/x) improved by 60?% when compared with that noted in the control. These results demonstrate that l-serine production in C. glutamicum SYPS-062 could be improved by overexpressing a C-terminal truncation of PGDH in combination with other genetic modifications.