Detected microsatellite polymorphisms in genetically altered inbred mouse strains

详细信息    查看全文

• 作者：Xiaoyan Du ; Jing Cui ; Chao Wang ; Xueyun Huo ; Jing Lu…
• 关键词：Microsatellite ; Mutation ; Transgenic mice ; Gene knockout mice ; ENU ; treated mice
• 刊名：Molecular Genetics and Genomics
• 出版年：2013
• 出版时间：August 2013
• 年：2013
• 卷：288
• 期：7-8
• 页码：309-316
• 全文大小：188KB
• 参考文献：1. Beal MA, Glenn TC, Lance SL, Somers CM (2000) Characterization of unstable microsatellites in mice: no evidence for germline mutation induction following gamma-radiation exposure. Environ Mol Mutagen 53:599-07 CrossRef
  2. Chang EY, Dorsey PB, Johnson N et al (2006) A prospective analysis of microsatellite instability as a molecular marker in colorectal cancer. Am J Surg 191:646-51 CrossRef
  3. Clark KJ, Carlson DF, Foster LK et al (2007) Enzymatic engineering of the porcine genome with transposons and recombinases. BMC Biotechnol 7:42. doi:10.1186/1472-6750-7-42 CrossRef
  4. Du XY, Chen ZW, Li W et al (2010) Development of novel microsatellite DNA markers by cross-amplification and analysis of genetic variation in gerbils. J Hered 101:710-16 CrossRef
  5. Eckert KA, Hile SE (1998) Alkylation-induced frameshift mutagenesis during in vitro DNA synthesis by DNA polymerases alpha and beta. Mutat Res Fundmolm 422:255-69 CrossRef
  6. El-Ghor AA, Noshy MM, El Ashmaoui HM et al (2010) Microsatellite instability at three microsatellite loci (D6mit3, D9mit2 and D15Mgh1) located in different common fragile sites of rats exposed to cadmium. Mutat Res Gene Tox En 696:160-66 CrossRef
  7. Garber JC, Barbee RW, TBielitzki J (2011) Guide for the care and use of laboratory animals, 8th edn. The National Academies Press, USA
  8. Gordon JW (1989) Transgenic animals. Int Rev Cytol 115:171-29 CrossRef
  9. Habano W, Sugai T, Nakamura S et al (2000) Microsatellite instability and mutation of mitochondrial and nuclear DNA in gastric carcinoma. Gastroenterology 118:835-41 CrossRef
  10. Hau J, Schapiro SJ (2011) Handbook of laboratory animal science, 3rd edn. CRC Press, Taylor & Francis Group, USA
  11. Healy C, Wade M, McMahon A et al (2006) Flow cytometric detection of tandem repeat mutations induced by various chemical classes. Mutat Res Fundmolm 598:85-02 CrossRef
  12. Hite JM, Eckert KA, Cheng KC (1996) Factors affecting fidelity of DNA synthesis during PCR amplification of d(C-A)(n)center dot d(G-T)(n) microsatellite repeats. Nucleic Acids Res 24:2429-434 CrossRef
  13. Jansen L, Claij N, Dekker M et al (2000) Acceleration of lymphomagenesis in mismatch-repair deficient mice by exposure to genotoxic agents. Toxicol Lett 112:245-50 CrossRef
  14. Justice MJ, Carpenter DA, Favor J et al (2000) Effects of ENU dosage on mouse strains. Mamm Genome 11:484-88 CrossRef
  15. Kim WS, Park C, Hong SK et al (2000) Microsatellite instability (CMP) in non-small cell lung cancer (NSCLC) is highly associated with transforming growth factor-beta type II receptor (TGF-beta RII) frameshift mutation. Anticancer Res 20:1499-502
  16. Klapacz J, Lingaraju GM, Guo HH et al (2010) Frameshift mutagenesis and microsatellite instability induced by human alkyladenine DNA glycosylase. Mol Cell 37:843-53 CrossRef
  17. Kruglyak S, Durrett R, Schug MD, Aquadro CF (2000) Distribution and abundance of microsatellites in the yeast genome can be explained by a balance between slippage events and point mutations. Mol Biol Evol 17:1210-219 CrossRef
  18. Levinson G, Gutman GA (1987) Slipped-strand mispairing—a major mechanism for DNA-sequence evolution. Mol Biol Evol 4:203-21
  19. Ma ZQ, Roder M, Sorrells ME (1996) Frequencies and sequence characteristics of di-, tri-, and tetra-nucleotide microsatellites in wheat. Genome 39:123-30 CrossRef
  20. Martin P, Makepeace K, Hill SA et al (2005) Microsatellite instability regulates transcription factor binding and gene expression. Proc Natl Acad Sci USA 102:3800-804 CrossRef
  21. Monica J, Justice JK, Noveroske JSW et al (1999) Mouse ENU mutagenesis. Hum Mol Genet 8:1955-963 CrossRef
  22. Moorehead RA, Sanchez OH, Baldwin RM, Khokha R (2003) Transgenic over expression of IGF-II induces spontaneous lung tumors: a model for human lung adenocarcinoma. Oncogene 22:853-57 CrossRef
  23. Mumphrey SM, Changotra H, Moore TN et al (2007) Murine norovirus 1 infection is associated with histopathological changes in immunocompetent hosts, but clinical disease is prevented by STAT1-dependent interferon responses. J Virol 81:3251-263 CrossRef
  24. Sara I, Jenny C, Viktor K et al (2008) Huntington’s disease: from pathology and genetics to potential therapies. Biochem J 412:191-09 CrossRef
  25. Schlotterer C (2000) Evolutionary dynamics of microsatellite DNA. Chromosoma 109:365-71 CrossRef
  26. Tosal L, Comendador MA, Sierra LM (2001) In vivo repair of ENU-induced oxygen alkylation damage by the nucleotide excision repair mechanism in Drosophila melanogaster. Mol Genet Genom 265:327-35 CrossRef
  27. Toth G, Gaspari Z, Jurka J (2000) Microsatellites in different eukaryotic genomes: survey and analysis. Genome Res 10:967-81 CrossRef
  28. Weber JL, Wong C (1993) Mutation of human short tandem repeats. Hum Mol Genet 2:1123-128 CrossRef
  29. Zuo B, Du X, Zhao J et al (2012) Analysis of microsatellite polymorphism in inbred knockout mice. PLoS One 7:e34555. doi:10.1371/journal.pone.0034555 CrossRef
  
• 作者单位：Xiaoyan Du (1)
  Jing Cui (1)
  Chao Wang (1)
  Xueyun Huo (1)
  Jing Lu (1)
  Yichen Li (1)
  Zhenwen Chen (1)
  
  1. Department of Laboratory Animal Science, School of Basic Medical Science, Capital Medical University, Beijing, 100069, China
  
• ISSN：1617-4623

文摘

Microsatellites are 50-00 repetitive DNA sequences composed of 1- to 6-base-pair-long reiterative motifs within the genome. They are vulnerable to DNA modifications, such as recombination and/or integration, and are recognized as “sentinel-DNA. Our previous report indicated that the genotypes of the microsatellite loci could change from mono- to poly-morphisms (CMP) in gene knockout (KO) mice, implying that genetic modification induces microsatellite mutation. However, it is still unclear whether the random insertion of DNA fragments into mice genomes produced via transgene (Tg) or N-ethyl-N-nitrosourea (ENU) would also result in microsatellite mutations or microsatellite loci genotypes changes. This study was designed to find possible clues to answer this question. In brief, 198 microsatellite loci that were distributed among almost all of the chromosomes (except for the Y) were examined through polymerase chain reaction to screen possible CMPs in six Tg strains. First, for each strain, the microsatellite sequences of all loci were compared between Tg and the corresponding background strain to exclude genetic interference. Simultaneously, to exclude spontaneous mutation-related CMPs that might exist in the examined six strains, mice from five spontaneously mutated inbred strains were used as the negative controls. Additionally, the sequences of all loci in these spontaneous mutated mice were compared to corresponding genetic background controls. The results showed that 40 of the 198 (20.2?%) loci were identified as having CMPs in the examined Tg mice strains. The CMP genotypes were either homozygous or heterozygous compared to the background controls. Next, we applied the 40 CMP positive loci in ENU-mutated mice and their corresponding background controls. After that, a general comparison of CMPs that exist among Tg, ENU-treated and KO mouse strains was performed. The results indicated that four (D11mit258, D13mit3, D14mit102 and DXmit172) of the 40 (10?%) CMP loci were shared by Tg and KO mice, two (D15mit5 and D14mit102) (5?%) by Tg and ENU-treated mice, and one (D14mit102) (2.5?%) by all three genetic modifications. Collectively, our study implies that genetic modifications by KO, Tg or chemical mutant can trigger microsatellite CMPs in inbred mouse strains. These shared microsatellite loci could be regarded as “hot spots-of microsatellite mutation for genetic monitoring in genetic modified mice.