BMP9-induced osteogenic differentiation is partially inhibited by miR-30a in the mesenchymal stem cell line C3H10T1/2

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• 作者：Ruyi Zhang ; Yaguang Weng ; Baolin Li ; Yingjiu Jiang…
• 关键词：Bone morphogenetic protein 9 ; MiR ; 30a ; Runx2 ; Osteogenic differentiation ; Mesenchymal stem cells
• 刊名：Journal of Molecular Histology
• 出版年：2015
• 出版时间：October 2015
• 年：2015
• 卷：46
• 期：4-5
• 页码：399-407
• 全文大小：5,365 KB
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• 作者单位：Ruyi Zhang (1)
  Yaguang Weng (1)
  Baolin Li (2)
  Yingjiu Jiang (3)
  Shujuan Yan (1)
  Fang He (1)
  Xiaoqing Chen (4)
  Fang Deng (1)
  Jing Wang (1)
  Qiong Shi (1)
  
  1. Key Laboratory of Diagnostic Medicine Designated by the Chinese Ministry of Education, Department of Laboratory Medicine, Chongqing Medical University, 1 Yixueyuan Road, Chongqing, 400016, China
  2. Department of Laboratory Medicine, Affiliated Hospital of Luzhou Medical College, Luzhou City, 646000, Sichuan, China
  3. The First Affiliated Hospital, Chongqing Medical University, Chongqing, 400016, China
  4. Department of Pathology, Southwest Hospital, Third Military Medical University, Chongqing, 400038, China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Cell Biology
  Biomedicine
  Developmental Biology
  
• 出版者：Springer Netherlands
• ISSN：1567-2387

文摘

In the bone morphogenetic protein (BMP) family, BMP9 is the strongest inducer of osteogenic differentiation in mesenchymal stem cells. Recent studies have suggested that the miR-30 family regulates cell proliferation and osteoblastic differentiation. In the present study, we found that expression of only one miR-30 family member, miR-30a, first decreased and then increased during BMP9-induced osteogenic differentiation. Cell proliferation assays revealed that miR-30a had no effect on the proliferation of C3H10T1/2 cells. However, over-expression of miR-30a led to expression of an early osteogenic marker and a reduction in Runx2 expression. In addition, we observed decreases in the expression of late osteogenic markers and osteopontin, as well as calcium deposition. Dual-luciferase reporter assays indicated that this process might be mediated by suppressing Runx2 protein expression. In vivo stem cell implantation revealed inhibition of BMP9-induced ectopic bone formation and matrix mineralization by miR-30a. This study provides a better understanding of the molecular mechanisms through which miR-30a negatively regulates BMP9-induced osteogenic differentiation.