Bio-inspired peptide-Au cluster applied for mercury (II) ions detection

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• 作者：Yaling Wang ; Yanyan Cui ; Ru Liu ; Fuping Gao ; Liang Gao…
• 关键词：peptide ; Au cluster ; mercury (II) ions ; fluorescence ; detection
• 刊名：SCIENCE CHINA Chemistry
• 出版年：2015
• 出版时间：May 2015
• 年：2015
• 卷：58
• 期：5
• 页码：819-824
• 全文大小：946 KB
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• 作者单位：Yaling Wang (1)
  Yanyan Cui (1) (2)
  Ru Liu (1)
  Fuping Gao (1)
  Liang Gao (1)
  Xueyun Gao (1)
  
  1. Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety; Institute of High Energy Physics, Chinese Academy of Sciences, Beijing, 100049, China
  2. College of Materials Science and Opto-electronic Technology, University of Chinese Academy of Sciences, Beijing, 100049, China
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Chinese Library of Science
  Chemistry
  
• 出版者：Science China Press, co-published with Springer
• ISSN：1869-1870

文摘

Mercury ion (Hg2+ ) pollution exists in water, soil, and food. By interacting with the thiol groups in protein, Hg2+ ions can accumulate in ways that cause serious damage to the central nervous system and threaten human health and natural environment. Undoubtedly, Hg2+ ion detection is a significant issue in environment and health monitoring. A variety of sensor platforms for Hg2+ ion detection based on organic molecules, DNA, oligonucleotides, inorganic materials, etc, have been reported. In this paper, an artificial peptide PHg, with a cluster bio-mineralize sequence (CCY) and a multi-charge hydrophilic sequence is designed as a template for the one-step synthesis of a peptide-Au cluster probe. Briefly: the peptide PHg in situ anchors Au ions to form a peptide-Au (I) intermediate and the reaction pH with NaOH is adjusted; after 12 h incubation at room temperature, the peptide PHg-Au nanocluster probe with red fluorescence is obtained. The probe has a super-small core size of approximately 1.26 nm and a maximum emission peak at 650 nm. The presence of Hg2+ ions cause the fluorescence of the probe to greatly decrease. Based on the differences in fluorescence intensity of the PHg-Au nanocluster in the absence and presence of Hg2+ ions, Hg2+ ions could be quantitatively detected in concentrations ranging from 5 nmol/L to 1 μmol/L. The limit of detection (LOD) is 7.5 nmol/L. Compared with some interference ions such as, K+, Mg2+, Ca2+, Pb2+, Ni2+, Fe3+, and Cu2+, the selectivity was excellent. The sensing of Hg2+ ion is not affected by the chelate agents: EDTA, which imparts a significant advantage in a range of applications. As a result, a simple, sensitive and selective fluorescent assay based on peptide PHg-Au cluster is developed for the detection of Hg2+ ions.