A novel nanocomposite matrix based on graphene oxide and ferrocene-branched organically modified sol–gel/chitosan for biosensor application
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- 作者：Huaping Peng (1) (2)
Zhengjun Huang (2)
Yanjie Zheng (1) (2)
Wei Chen (1) (2)
Ailin Liu (1) (2)
Xinhua Lin (1) (2) - 关键词：Graphene oxide ; Chitosan ; Ferrocene ; branched organically modified sol–gel ; Glucose oxidase ; Biosensor
- 刊名：Journal of Solid State Electrochemistry
- 出版年：2014
- 出版时间：July 2014
- 年：2014
- 卷：18
- 期：7
- 页码：1941-1949
- 全文大小：1,040 KB
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Zhengjun Huang (2)
Yanjie Zheng (1) (2)
Wei Chen (1) (2)
Ailin Liu (1) (2)
Xinhua Lin (1) (2)
1. Department of Pharmaceutical Analysis, Faculty of Pharmacy, Fujian Medical University, Fuzhou, 350004, China
2. Nano Medical Technology Research Institute, Fujian Medical University, Fuzhou, 350004, China - ISSN：1433-0768
文摘
A novel platform for the fabrication of a glucose biosensor was successfully constructed by entrapping glucose oxidase (GOD) in a ferrocene (Fc)-branched organically modified silica material (ormosil)/chitosan (CS)/graphene oxide (GO) nanocomposite. The morphology, structure, and electrochemistry of the nanocomposite were characterized by transmission electron microscopy, X-ray powder diffraction, UV–vis spectroscopy, Fourier transform infrared spectroscopy, and electrochemical techniques. Results demonstrated that the proposed electrochemical platform not only provided an excellent microenvironment to maintain the bioactivity of the immobilized enzyme, but also effectively prevented the leakage of both the enzyme and mediator from the matrix and retained the electrochemical activity of Fc. Furthermore, dispersing GO within the Fc-branched ormosil/CS matrix could significantly improve the stability of GO and make it exhibit a positive charge, which was more favorable for the further immobilization of biomolecules, such as GOD, with higher loading. Moreover, it could also improve the conductivity of the matrix film and facilitate the electron shuttle between the mediator and electrode. Under optimal conditions, the designed biosensor to glucose exhibited a wide and useful linear range of 0.02 to 5.39?mM with a low detection limit of 6.5?μM. The value of K M app was 4.21?mM, indicating that the biosensor possesses higher biological affinity to glucose. The present approach could be used efficiently for the linkage of other redox mediators and immobilize other biomolecules in the process of fabricating novel biosensors.