Genetic analysis of the Holliday junction resolvases Hje and Hjc in Sulfolobus islandicus

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• 作者：Qihong Huang ; Yansheng Li ; Chaoning Zeng ; Tengteng Song ; Zhou Yan…
• 关键词：Archaea ; Holliday junction resolvase ; Hje ; Hjc ; DNA repair
• 刊名：Extremophiles
• 出版年：2015
• 出版时间：March 2015
• 年：2015
• 卷：19
• 期：2
• 页码：505-514
• 全文大小：709 KB
• 参考文献：1. Aravind, L, Makarova, KS, Koonin, EV (2000) Survey and summary: Holliday junction resolvases and related nucleases: identification of new families, phyletic distribution and evolutionary trajectories. Nucleic Acids Res 28: pp. 3417-3432 CrossRef
  2. Bond, CS, Kvaratskhelia, M, Richard, D, White, MF, Hunter, WN (2001) Structure of Hjc, a Holliday junction resolvase, from Sulfolobus solfataricus. Proc Natl Acad Sci USA 98: pp. 5509-5514 CrossRef
  3. Chan, YW, West, SC (2014) Spatial control of the GEN1 Holliday junction resolvase ensures genome stability. Nat Commun 5: pp. 4844 CrossRef
  4. Deng, L, Zhu, H, Chen, Z, Liang, YX, She, Q (2009) Unmarked gene deletion and host-vector system for the hyperthermophilic crenarchaeon Sulfolobus islandicus. Extremophiles 13: pp. 735-746 CrossRef
  5. Dorazi, R, Parker, JL, White, MF (2006) PCNA activates the Holliday junction endonuclease Hjc. J Mol Biol 364: pp. 243-247 CrossRef
  6. Dunderdale, HJ, Benson, FE, Parsons, CA, Sharples, GJ, Lloyd, RG, West, SC (1991) Formation and resolution of recombination intermediates by E. coli RecA and RuvC proteins. Nature 354: pp. 506-510 CrossRef
  7. Fekairi, S (2009) Human SLX4 is a Holliday junction resolvase subunit that binds multiple DNA repair/recombination endonucleases. Cell 138: pp. 78-89 CrossRef
  8. Fujikane, R, Ishino, S, Ishino, Y, Forterre, P (2010) Genetic analysis of DNA repair in the hyperthermophilic archaeon, Thermococcus kodakaraensis. Genes Genet Syst 85: pp. 243-257 CrossRef
  9. Guo, L (2011) Genome analyses of Icelandic strains of Sulfolobus islandicus, model organisms for genetic and virus–host interaction studies. J Bacteriol 193: pp. 1672-1680 CrossRef
  10. Heyer, WD, Ehmsen, KT, Solinger, JA (2003) Holliday junctions in the eukaryotic nucleus: resolution in sight?. Trends Biochem Sci 28: pp. 548-557 CrossRef
  11. Hong Y et al. (2011) Dissection of the functional domains of an archaeal Holliday junction helicase. DNA Repair (Amst)
  12. Ip, SC, Rass, U, Blanco, MG, Flynn, HR, Skehel, JM, West, SC (2008) Identification of Holliday junction resolvases from humans and yeast. Nature 456: pp. 357-361 CrossRef
  13. Komori, K, Sakae, S, Shinagawa, H, Morikawa, K, Ishino, Y (1999) A Holliday junction resolvase from Pyrococcus furiosus: functional similarity to Escherichia coli RuvC provides evidence for conserved mechanism of homologous recombination in Bacteria, Eukarya, and Archaea. Proc Natl Acad Sci USA 96: pp. 8873-8878 CrossRef
  14. Kvaratskhelia, M, White, MF (2000) An archaeal Holliday junction resolving enzyme from Sulfolobus solfataricus exhibits unique properties. J Mol Biol 295: pp. 193-202 CrossRef
  15. Kvaratskhelia, M, White, MF (2000) Two Holliday junction resolving enzymes in Sulfolobus solfataricus. J Mol Biol 297: pp. 923-932 CrossRef
  16. Lestini, R, Duan, Z, Allers, T (2010) The archaeal Xpf/Mus81/FANCM homolog Hef and the Holliday junction resolvase Hjc define alternative pathways that are essential for cell viability in Haloferax volcanii. DNA Repair 9: pp. 994-1002 CrossRef
  17. Li, X (2011) Deletion of the topoisomerase III gene in the hyperthermophilic archaeon Sulfolobus
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Microbiology
  Biotechnology
  Ecology
  
• 出版者：Springer Japan
• ISSN：1433-4909

文摘

The in vivo functions of Hje and Hjc, two Holliday junction resolvases in Sulfolobus islandicus were investigated. We found that deletion of either hje or hjc had no effect on normal cell growth, while deletion of both hje and hjc is lethal. Although Hjc is the conserved resolvase in all archaea, the hje deletion rather than hjc deletion rendered cells more sensitive to DNA-damaging agents such as hydroxyurea, cisplatin, and methyl methanesulfonate than the wild type (WT). Intriguingly, the sensitivity of Δhje could not be rescued by ectopic expression of Hje from a plasmid and Hje overexpression slowed growth and large cells appeared with more than two genome equivalents. We showed that Hje was maintained at a low level in WT cells. Furthermore, transcriptomic microarray analysis revealed that the abundance of transcripts of many genes including those involved in DNA replication, repair, transcription regulation, and cell division changed drastically in the Hje-overexpressed strain. However, only limited genes were up- or downregulated in the hje deletion strain. Our findings collectively suggest that Hje is the primary resolvase involved in DNA repair and its expression must be tightly controlled in cells.