Development and characterization of 42 novel polymorphic microsatellite markers for Odontobutis potamophila from EST sequences

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• 作者：Lijuan Zhang (1) (2)
  Hongwei Zhang (1) (2)
  Yaping Zhang (1) (2)
  Fei Zhu (1) (2)
  Xinyuan Hou (1) (2)
  Yali Hu (1) (2)
  Shaowu Yin (1) (2)
  
• 关键词：Odontobutis potamophila ; Expressed sequence tags (EST) ; Microsatellites
• 刊名：Conservation Genetics Resources
• 出版年：2014
• 出版时间：June 2014
• 年：2014
• 卷：6
• 期：2
• 页码：469-472
• 全文大小：
• 参考文献：1. Gao ZX, Luo W, Liu H, Zeng C, Liu XL, Yi SK, Wang WM (2012) Transcriptome analysis and SSR/SNP markers information of the blunt snout bream ( / Megalobrama amblycephala). PLoS ONE 7(8):1鈥?0
  2. Huckstorf V (2012) / Odontobutis potamophilus. In: IUCN 2012. IUCN Red List of Threatened Species
  3. Oosterhout CV, Hutchinson WF, Wills DPM, Shipley P (2004) MICRO-CHECKER: software for identifying and correcting genotyping errors in microsatellite data. Mol Ecol Notes 4:535鈥?38 CrossRef
  4. Schuelke M (2000) An economic method for the use of fluorescent labeling of PCR fragments. Nat Biotech 18:233鈥?34 CrossRef
  5. Yeh F, Yang RC, Boyle T (1999) POPGENE v3.2, http://www.ualberta.ca/~fyeh/download.htm
  
• 作者单位：Lijuan Zhang (1) (2)
  Hongwei Zhang (1) (2)
  Yaping Zhang (1) (2)
  Fei Zhu (1) (2)
  Xinyuan Hou (1) (2)
  Yali Hu (1) (2)
  Shaowu Yin (1) (2)
  
  1. Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing, 210023, China
  2. Jiangsu Co-Innovation Center for Marine Bio-Industry Technology, Lianyungang, 222005, China
  
• ISSN：1877-7260

文摘

Expressed sequence tags (ESTs) are sources for microsatellite development. In this study, we isolated and characterized microsatellite makers for Odontobutis potamophila using next-generation sequencing. A total of 42 polymorphic microsatellite repeat markers were identified in 32 individuals from Dangtu wild population. The number of alleles per locus varied from 2 to 10, and the observed and expected heterozygosity ranged from 0.000 to 1.000 and from 0.061 to 0.878, respectively. These EST-SSR makers would provide sufficient polymorphism for population genetic studies, parentage and genome mapping of O. potamophila and its closely related fishes.