Vasorelaxing and antihypertensive activities of synthesized peptides derived from computer-aided simulation of pepsin hydrolysis of yam dioscorin

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• 作者：Yin-Shiou Lin ; Yeh-Lin Lu ; Guei-Jane Wang ; Hong-Jen Liang ; Wen-Chi Hou
• 关键词：Angiotensin converting enzyme ; Antihypertensive activity ; Blood pressure ; Vasorelaxing ; Yam dioscorin
• 刊名：Botanical Studies
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：55
• 期：1
• 全文大小：593 KB
• 参考文献：1. Cheung, HS, Wang, FL, Ondetti, MA, Sabo, EF, Cushman, DW (1980) Binding of peptide substrates and inhibitors of angiotensin-converting enzyme. Importance of the COOH-terminal dipeptide sequence. J Biol Chem 255: pp. 401-407
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  6. Han, CH, Lin, YS, Lin, SY, Hou, WC (2014) Antioxidant and antiglycation activities of the synthesised dipeptide, Asn-Trp, derived from computer-aided simulation of yam dioscorin hydrolysis and its analogue, Gln-Trp. Food Chem 147: pp. 195-202 CrossRef
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• 刊物主题：Plant Sciences;
• 出版者：Springer Berlin Heidelberg
• ISSN：1999-3110

文摘

Background We reported that yam dioscorin and its peptic hydrolysates exhibited ACE inhibition and antihypertensive effects on SHRs, however, the active peptides are not really isolated until now. Using ACE inhibitory screenings, two penta-peptides, KTCGY and KRIHF, were selected for ex vivo and in vivo experiments. Results KTCGY, KRIHF, and captopril were shown to have similar vasodilating effects against phenylephrine (PE)-induced tensions in rat endothelium-dependent thoracic aortic rings, however, KTCGYKTCGY (two-repeated KTCGY) and TCGYTCGY (two-repeated TCGY) were showed endothelium-independent vasodilating effects against PE-induced tensions. KTCGY, KRIHF (10 or 20?mg/kg), and captopril (10?mg/kg) were used to evaluate antihypertensive activity during 24-h after a single oral administration to spontaneously hypertensive rats (SHRs). The KTCGY and KRIHF showed significantly different and reduced the systolic blood pressure of SHRs compared to the blank. Conclusions These results suggest that KTCGY and KRIHF may contribute important roles in yam dioscorin for regulating blood pressure in vivo.