Genome analysis of Enterococcus faecalis bacteriophage IME-EF3 harboring a putative metallo-beta-lactamase gene

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• 作者：Xiaoyu Li (1) (2)
  Peng Ding (2)
  Chuanyin Han (1)
  Hang Fan (1)
  Yahui Wang (1)
  Zhiqiang Mi (1)
  Fumin Feng (2)
  Yigang Tong (1)
  
• 关键词：Bacteriophage ; Complete genome sequence ; Enterococcus faecalis ; Metallo ; beta ; lactamase
• 刊名：Virus Genes
• 出版年：2014
• 出版时间：August 2014
• 年：2014
• 卷：49
• 期：1
• 页码：145-151
• 全文大小：3,788 KB
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• 作者单位：Xiaoyu Li (1) (2)
  Peng Ding (2)
  Chuanyin Han (1)
  Hang Fan (1)
  Yahui Wang (1)
  Zhiqiang Mi (1)
  Fumin Feng (2)
  Yigang Tong (1)
  
  1. Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing, 100071, China
  2. School of Public Health, Hebei United University, Tangshan, 063000, Hebei, China
  
• ISSN：1572-994X

文摘

Lytic Enterococcus faecalis bacteriophage IME-EF3 was isolated from hospital sewage, and its genome was sequenced using high-throughput sequencing. Genomic analysis and electron microscopy suggested that IME-EF3 was a member of the family Siphoviridae. The phage has an isometric head and a long non-contractile tail with a 41?kb linear double-stranded DNA genome. The genome encodes 69 putative proteins, with 32 annotated functionally, including proteins related to phage structure, packaging, transcription, replication, and a lysis module. Interestingly, a metallo-beta-lactamase gene responsible for multi-drug resistance was found in the genome of IME-EF3. The possibility of horizontal gene transfer of the metallo-beta-lactamase gene suggests that phage IME-EF3, although lytic, might not be suitable for phage therapy unless one would devise a way to delete the metallo-beta-lactamase gene. Hence, whole genome sequencing should always be a prerequisite for identifying a phage therapy candidate.