Microsphere-based antibody assays for human parvovirus B19V, CMV and T. gondii

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• 作者：Yilin Wang ; Lea Hedman ; Maria F. Perdomo ; Amal Elfaitouri…
• 关键词：Microsphere ; based suspension immuno assay ; Singleplex and multiplex ; B19V ; CMV ; T. gondii ; Pregnancy
• 刊名：BMC Infectious Diseases
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：16
• 期：1
• 全文大小：532 KB
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• 作者单位：Yilin Wang (1)
  Lea Hedman (2)
  Maria F. Perdomo (1)
  Amal Elfaitouri (3)
  Agnes Bölin-Wiener (3)
  Arun Kumar (1) (4)
  Maija Lappalainen (1) (2)
  Maria Söderlund-Venermo (1)
  Jonas Blomberg (3)
  Klaus Hedman (1) (2)
  
  1. Virology, University of Helsinki, Haartmaninkatu 3, FI-00290, Helsinki, Finland
  2. Helsinki University Hospital, Helsinki, Finland
  3. Section of Clinical Virology, Department of Medical Sciences, Uppsala University and Uppsala University Hospital, Uppsala, Sweden
  4. Present address: T-Cell Platform, Translational Medicine, Novartis Vaccines (a GSK company), Siena, Italy
  
• 刊物主题：Infectious Diseases; Parasitology; Medical Microbiology; Tropical Medicine; Internal Medicine;
• 出版者：BioMed Central
• ISSN：1471-2334

文摘

Background Human parvovirus B19 (B19V), cytomegalovirus (CMV) and Toxoplasma gondii (T. gondii) may cause intrauterine infections with potentially severe consequences to the fetus. Current serodiagnosis of these infections is based on detection of antibodies most often by EIA and individually for each pathogen. We developed singleplex and multiplex microsphere-based Suspension Immuno Assays (SIAs) for the simultaneous detection of IgG antibodies against B19V, CMV and T. gondii.