A hydrogen peroxide biosensor based on multiwalled carbon nanotubes-polyvinyl butyral film modified electrode
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- 作者：Li Han ; Han Tao ; Minsheng Huang ; Yiming Zhang…
- 关键词：biosensor ; horseradish peroxidase ; carbon nanotubes ; polyvinyl butyral ; hydrogen peroxide
- 刊名：Russian Journal of Electrochemistry
- 出版年：2016
- 出版时间：February 2016
- 年：2016
- 卷：52
- 期：2
- 页码：115-122
- 全文大小：752 KB
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Han Tao (2)
Minsheng Huang (1)
Yiming Zhang (2) (3)
Shunbin Qiao (3)
Ruili Shi (3)
1. College of Resource and Environmental Sciences, East China Normal University, Shanghai, 200241, P. R. China
2. Province Key Laboratory of Fermentation Engineering and Biopharmaceutical, Guizhou University, Guiyang, 550003, P. R. China
3. Guizhou Industry Polytechnic College, Guiyang, 550008, P. R. China - 刊物类别：Chemistry and Materials Science
- 刊物主题：Chemistry
Electrochemistry
Physical Chemistry
Russian Library of Science - 出版者：MAIK Nauka/Interperiodica distributed exclusively by Springer Science+Business Media LLC.
- ISSN：1608-3342
文摘
A novel approach to construct a amperometric biosensor for determination of H2O2 is described. Horseradish peroxidase (HRP) as a base enzyme was immobilized into the mixture of multiwalled carbon nanotubes (MWNTs) and polyvinyl butyral (PVB). Taking the classical hydroquinone as mediator, cyclic voltammetry and amperometric measurements were used to study and optimize the performance of the resulting H2O2 biosensor. The effect of the concentration of MWNTs, HRP, hydroquinone, solution pH, and the working potential of amperometry on the electrochemical biosensor was systematically studied. The results showed that the fabricated biosensor demonstrated significant electrocatalytic activity for the reduction of hydrogen peroxide with wide linear range from 0.000832 to 0.6 mM, and low detection limit 0.000167 mM (S/N = 3) with fast response time less than 8 s. The apparent Michaelis–Menten constant was determined to be 0.049 mM. Additionally, the biosensor exhibited high sensitivity, rapid response and good long-term stability.