Cyclophilin A from Schistosoma japonicum promotes a Th2 response in mice

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• 作者：Jinghui Li (6)
  Wenjia Zhuang (6)
  Li Cong (6)
  Wenjun Shi (7)
  Xingyan Cai (6)
  Fengjuan Huang (6)
  Yiteng Liao (6)
  Yiyang Liu (8)
  Jun Li (6)
  Chunxia Chen (6)
  Xiao-Ping Chen (6)
  
• 关键词：Th2 response ; Schistosoma japonicum ; Cyclophilin A
• 刊名：Parasites & Vectors
• 出版年：2013
• 出版时间：December 2013
• 年：2013
• 卷：6
• 期：1
• 全文大小：611 KB
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• 作者单位：Jinghui Li (6)
  Wenjia Zhuang (6)
  Li Cong (6)
  Wenjun Shi (7)
  Xingyan Cai (6)
  Fengjuan Huang (6)
  Yiteng Liao (6)
  Yiyang Liu (8)
  Jun Li (6)
  Chunxia Chen (6)
  Xiao-Ping Chen (6)
  
  6. Department of Immunology, Tongji University School of Medicine, Shanghai, 200092, China
  7. Department of Pathogen Biology, Tongji University School of Medicine, Shanghai, 200092, China
  8. Shanghai Institute of Pharmaceutical Industry, Shanghai, 200040, China
  
• ISSN：1756-3305

文摘

Background Schistosomiasis is a chronic infection, where the host immune response to the parasite changes from a predominantly Th1 to Th2 phenotype, when parasite enters the egg stage, restraining the host inflammatory immune responses to achieve a longer survival in the host. On the other hand, the development of Th2 responses causes immunopathological changes such as liver fibrosis. Therefore identification of schistosome-derived Th2 inducing molecules is important in the understanding of pathogenesis of schistosomiasis. A cyclophilin A homologue of Schistosoma japonicum was reported to be an egg-stage specific antigen, but its immunogenicity and immunoregulatory activities remain unknown. Methods We cloned and expressed the gene of cyclophilin A from Schistosoma japonicum (AY814078), named as SjCyP18 based on its molecular weight. The expression profiles in different stages of S. japonicum were examined by RT-PCR and immunofluorescence assay. The immunogenicity of SjCyP18 was measured by the presence of IgG in the sera from S. japonicum infected patients and animals, and the Th2-promting activities were examined by the subclass of immunoglobulins against SjCyP18 and by the IL-4 induction in T cells following footpad injection of SjCyP18. Results The cloned SjCyP18 has 65% homology with human or mouse cyclophilin A at the amino acid level. In contrast to reports as an egg-stage specific antigen, the gene was found to be expressed in all stages of S. japonicum. IgG responses against SjCyP18 were found in some S. japonicum infected patients and were significantly induced when infection become patent and produce eggs in infected mice. Furthermore, the Th2-promoting subclass of IgG1 was the predominant isotype in S. japonicum infected mice. More importantly, footpad injection of SjCyP18 induced a greater production of IL-4 than that of IFN-γ by lymphocytes compared to responses from PBS injection controls. Conclusion The cyclophilin A homologue found in S. japonicum is immunogenic and promotes Th2 responses in vivo which may contribute to the establishment of chronic infection by schistosomes.