Semi-industrial scale (30 m<sup class="a-plus-plus">3sup>) fed-batch fermentation for the production of d-lactate by Escherichia coli strain HBUT-D15

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• 作者：Xiangmin Fu ; Yongze Wang ; Jinhua Wang…
• 关键词：d ; lactic acid ; E. coli ; Fed ; batch fermentation ; Polylactic acid polymer ; Scale ; up fermentation
• 刊名：Journal of Industrial Microbiology & Biotechnology
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：44
• 期：2
• 页码：221-228
• 全文大小：
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Microbiology; Biochemistry, general; Inorganic Chemistry; Genetic Engineering; Biotechnology; Bioinformatics;
• 出版者：Springer Berlin Heidelberg
• ISSN：1476-5535
• 卷排序：44

文摘

d(−)-lactic acid is needed for manufacturing of stereo-complex poly-lactic acid polymer. Large scale d-lactic acid fermentation, however, has yet to be demonstrated. A genetically engineered Escherichia coli strain, HBUT-D, was adaptively evolved in a 15% calcium lactate medium for improved lactate tolerance. The resulting strain, HBUT-D15, was tested at a lab scale (7 L) by fed-batch fermentation with up to 200 g L−1 of glucose, producing 184–191 g L−1 of d-lactic acid, with a volumetric productivity of 4.38 g L−1 h−1, a yield of 92%, and an optical purity of 99.9%. The HBUT-D15 was then evaluated at a semi-industrial scale (30 m3) via fed-batch fermentation with up to 160 g L−1 of glucose, producing 146–150 g L−1 of d-lactic acid, with a volumetric productivity of 3.95–4.29 g L−1 h−1, a yield of 91–94%, and an optical purity of 99.8%. These results are comparable to that of current industrial scale l(+)-lactic acid fermentation.