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Breeding of cabbage (Brassica oleracea L. var. capitata) with fusarium wilt resistance based on microspore culture and marker-assisted selection
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  • 作者:Hong-hao Lv (1)
    Qing-biao Wang (1)
    Li-mei Yang (1)
    Zhi-yuan Fang (1)
    Yu-mei Liu (1)
    Mu Zhuang (1)
    Yang-yong Zhang (1)
    Yu-hong Yang (1)
    Bing-yan Xie (1)
    Xiao-wu Wang (1)
  • 关键词:Brassica oleracea ; Fusarium wilt ; Marker ; assisted selection ; Microspore culture ; Cabbage breeding
  • 刊名:Euphytica
  • 出版年:2014
  • 出版时间:December 2014
  • 年:2014
  • 卷:200
  • 期:3
  • 页码:465-473
  • 全文大小:1,461 KB
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  • 作者单位:Hong-hao Lv (1)
    Qing-biao Wang (1)
    Li-mei Yang (1)
    Zhi-yuan Fang (1)
    Yu-mei Liu (1)
    Mu Zhuang (1)
    Yang-yong Zhang (1)
    Yu-hong Yang (1)
    Bing-yan Xie (1)
    Xiao-wu Wang (1)

    1. Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, Beijing, 100081, China
  • ISSN:1573-5060
文摘
Cabbage Fusarium wilt (CFW), caused by Fusarium oxysporum f. sp. conglutinans, is one of the most devastating diseases of cabbage worldwide. In recent years, CFW has been spreading across northern Chinad. Thus, development of resistant cultivars is necessary to control CFW. Here we report microspore culture from a cross of two elite cabbage inbred lines, 96-00 (highly resistant to CFW) and 01-0 (highly susceptible to CFW), in order to obtain doubled haploids (DHs) to cultivate cabbage materials for resistance breeding. A total of 196 DHs were obtained in 2011 and 2012 and three elite DH lines were selected from them through agronomic trait evaluation as well as marker-assisted selection. These three elite DH lines were crossed with elite cabbage inbred lines to create hybrids, which were then evaluated for their resistance and agronomic traits. Three excellent hybrids were selected. The use of microspore culture and marker-assisted selection greatly shortened the time required to obtain these excellent hybrids. These resistant varieties may become an effective control of CFW in diseased areas.

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