Construction of over-expression shuttle vectors in Streptomyces
详细信息 查看全文
- 作者：Ning Sun (1)
Zhi-Bin Wang (1)
He-Ping Wu (2)
Xu-Ming Mao (1)
Yong-Quan Li (1) - 关键词：High ; copy number ; Constitutive ; Inducible ; pIJ101 replicon ; oriT
- 刊名：Annals of Microbiology
- 出版年：2012
- 出版时间：December 2012
- 年：2012
- 卷：62
- 期：4
- 页码：1541-1546
- 全文大小：310KB
- 参考文献：1. Ann J, van Mellaert L (1993) / Streptomyces lividans as Host for heterologous protein production. FEMS Microbiol Lett 114:121鈥?28 CrossRef
2. Bentley SD, Chater KF, Cerdeno-Tarraga AM, Challis GL, Thomson NR, James KD, Harris DE, Quail MA, Kieser H, Harper D, Bateman A, Brown S, Chandra G, Chen CW, Collins M, Cronin A, Fraser A, Goble A, Hidalgo J, Hornsby T, Howarth S, Huang CH, Kieser T, Larke L, Murphy L, Oliver K, O鈥橬eil S, Rabbinowitsch E, Rajandream MA, Rutherford K, Rutter S, Seeger K, Saunders D, Sharp S, Squares R, Squares S, Taylor K, Warren T, Wietzorrek A, Woodward J, Barrell BG, Parkhill J, Hopwood DA (2002) Complete genome sequence of the model actinomycete / Streptomyces coelicolor A3(2). Nature 417:141鈥?47 CrossRef
3. Bibb MJ, Janssen GR, Ward JM (1985) Cloning and analysis of the promoter region of the erythromycin resistance gene ( / ermE) of / Streptomyces erythraeus. Gene 38:215鈥?26 CrossRef
4. Hatanaka T, Onaka H, Arima J, Uraji M, Uesugi Y, Usuki H, Nishimoto Y, Iwabuchi M (2008) pTONA5: a hyperexpression vector in / Streptomycetes. Protein Expr Purif 62:244鈥?48 CrossRef
5. Janssen GR, Ward JM, Bibb MJ (1989) Unusual transcriptional and translational features of the aminoglycoside phosphotransferase gene ( / aph) from / Streptomyces fradiae. Genes Dev 3:415鈥?29 CrossRef
6. Kieser T, Bibb MJ, Butter MJ, Chater KF, Hopwood DA (2000) Practical / Streptomyces Genetics: The John Innes Foundation.
7. Kieser T, Hopwood DA, Wright HM, Thompson CJ (1982) pIJ101, a multi-copy broad host-range / Streptomyces plasmid: functional analysis and development of DNA cloning vectors. Mol Gen Genet 185:223鈥?28 CrossRef
8. Komeda H, Hori Y, Kobayashi M, Shimizu S (1996a) Transcriptional regulation of the / Rhodococcus rhodochrous J1 / nitA gene encoding a nitrilase. Proc Natl Acad Sci USA 93:10572鈥?0577 CrossRef
9. Komeda H, Kobayashi M, Shimizu S (1996b) A novel gene cluster including the / Rhodococcus rhodochrous J1 / nhlBA genes encoding a low molecular mass nitrile hydratase (L-NHase) induced by its reaction product. J Biol Chem 271:15796鈥?5802 CrossRef
10. MacNeil DJ, Occi JL, Gewain KM, MacNeil T, Gibbons PH, Ruby CL, Danis SJ (1992) Complex organization of the / Streptomyces avermitilis genes encoding the avermectin polyketide synthase. Gene 115:119鈥?25 CrossRef
11. Mao XM, Zhou Z, Cheng LY, Hou XP, Guan WJ, Li YQ (2009a) Involvement of SigT and RstA in the differentiation of / Streptomyces coelicolor. FEBS Lett 583:3145鈥?150 CrossRef
12. Mao XM, Zhou Z, Hou XP, Guan WJ, Li YQ (2009b) Reciprocal regulation between SigK and differentiation programs in / Streptomyces coelicolor. J Bacteriol 191:6473鈥?481 CrossRef
13. Paleckova P, Bobek J, Felsberg J, Mikulik K (2006) Activity of translation system and abundance of tmRNA during development of / Streptomyces aureofaciens producing tetracycline. Folia Microbiol 51:517鈥?24 CrossRef
14. Paleckova P, Felsberg J, Bobek J, Mikulik K (2007) tmRNA abundance in / Streptomyces aureofaciens, / S. griseus and / S. collinus under stress-inducing conditions. Folia Microbiol 52:463鈥?70 CrossRef
15. Pandey AK, Raman S, Proff R, Joshi S, Kang CM, Rubin EJ, Husson RN, Sassetti CM (2009) Nitrile-inducible gene expression in mycobacteria. Tuberculosis (Edinb) 89:12鈥?6 CrossRef
16. Sambrook J, MacCallum P, Russell D (2000) Molecular Cloning: A Laboratory Manual (Third Edition): Cold Spring Harbor Laboratory Press, NY
17. Schmitt-John T, Engels JW (1992) Promoter constructions for efficient secretion expression in / Streptomyces lividans. Appl Microbiol Biotechnol 36:493鈥?98 CrossRef
18. Sun J, Kelemen GH, Fernandez-Abalos JM, Bibb MJ (1999) Green fluorescent protein as a reporter for spatial and temporal gene expression in / Streptomyces coelicolor A3(2). Microbiology 145:2221鈥?227
19. Takano E, Gramajo HC, Strauch E, Andres N, White J, Bibb MJ (1992) Transcriptional regulation of the / redD transcriptional activator gene accounts for growth-phase-dependent production of the antibiotic undecylprodigiosin in / Streptomyces coelicolor A3(2). Mol Microbiol 6:2797鈥?804 CrossRef
20. Takano E, White J, Thompson CJ, Bibb MJ (1995) Construction of thiostrepton-inducible, high-copy-number expression vectors for use in / Streptomyces spp. Gene 166:133鈥?37 CrossRef
21. Withey JH, Friedman DI (2003) A salvage pathway for protein structures: tmRNA and trans-translation. Annu Rev Microbiol 57:101鈥?23 CrossRef
22. Yang C, Glover JR (2009) The SmpB-tmRNA tagging system plays important roles in / Streptomyces coelicolor growth and development. PLoS One 4:e4459 CrossRef
23. Yang R, Hu Z, Deng Z, Li J (1998) Construction of / Escherichia coli- / Streptomyces shuttle expression vectors for gene expression in / Streptomyces. Chin J Biotechnol 14:1鈥? - 作者单位：Ning Sun (1)
Zhi-Bin Wang (1)
He-Ping Wu (2)
Xu-Ming Mao (1)
Yong-Quan Li (1)
1. Institute of Biochemistry College of Life Sciences, Zhejiang University, Hangzhou, 310058, Peoples Republic of China
2. Jinqing Middle School, Luqiao, Taizhou, 318058, People鈥檚 Republic of China - ISSN：1869-2044
文摘
Two high-copy number vectors, pL97 and pL98, that contain the strong constitutive ermEp* from Saccharopolyspora erythraea and the ssrA promoter (ssrAp) from Streptomyces coelicolor, respectively, and an inducible high-copy vector, pL99, with the PnitA-NitR system from Rhodococcus rhodochrous, were constructed based on the pIJ101 replicon. These vectors have pUC18 and pIJ101 replication origins for high-copy plasmid number in Escherichia coli and Streptomyces, respectively, and the oriT (RK2) allows the efficient and convenient plasmid transfer from E. coli to Streptomyces. The transformants can be easily selected with apramycin. There is also a multiple cloning site (MCS) between promoter and terminator convenient for heterologous gene cloning. The enhanced green fluorescent protein (EGFP) gene and redD, a pathway-specific regulatory gene for the production of undecylprodigiosin in S. coelicolor, were inserted into these plasmids and could be over-expressed in S. coelicolor. Western blotting revealed that the expression of EGFP was dramatically increased compared to the cell with a single copy of EGFP. Furthermore, the production of undecylprodigiosin was also greatly enhanced in the cells constitutively over-expressing redD. Hence, the high-copy plasmids could be readily used to express foreign proteins and for production of secondary metabolites, especially the antibiotics, potentially for industrial applications.