Single Cas9 nickase induced generation of NRAMP1 knockin cattle with reduced off-target effects
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- 作者：Yuanpeng Gao ; Haibo Wu ; Yongsheng Wang ; Xin Liu ; Linlin Chen ; Qian Li…
- 关键词：CRISPR ; Cas9 ; Off ; target ; Homologous recombination ; Chromatin immunoprecipitation sequencing (ChIP ; seq) ; Nickase ; Single ; strand break ; Tuberculosis
- 刊名：Genome Biology
- 出版年：2017
- 出版时间：December 2017
- 年：2017
- 卷：18
- 期：1
- 全文大小：3039KB
- 刊物主题：Animal Genetics and Genomics; Human Genetics; Plant Genetics & Genomics; Microbial Genetics and Genomics; Bioinformatics; Evolutionary Biology;
- 出版者：BioMed Central
- ISSN：1474-760X
- 卷排序：18
文摘
BackgroundThe CRISPR-Cas9 system is a widely utilized platform for transgenic animal production in various species, although its off-target effects should be addressed. Several applications of this tool have been proposed in model animals but remain insufficient for transgenic livestock production.