An active hAT transposable element causing bud mutation of carnation by insertion into the flavonoid 3-/em>-hydroxylase gene

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• 作者：Masaki Momose (1)
  Masayoshi Nakayama (3)
  Yoshio Itoh (2)
  Naoyuki Umemoto (1)
  Toshihiro Toguri (1)
  Yoshihiro Ozeki (2)
  
• 关键词：hAT ; Active transposable element ; Bud mutation ; Flavonoid 3-hydroxylase ; Dianthus
• 刊名：Molecular Genetics and Genomics
• 出版年：2013
• 出版时间：4 - April 2013
• 年：2013
• 卷：288
• 期：3
• 页码：175-184
• 全文大小：424KB
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• 作者单位：Masaki Momose (1)
  Masayoshi Nakayama (3)
  Yoshio Itoh (2)
  Naoyuki Umemoto (1)
  Toshihiro Toguri (1)
  Yoshihiro Ozeki (2)
  
  1. Central Laboratories for Key Technologies, Kirin Co., Ltd, Fukuura 1-13-5, Yokohama, Kanagawa, 236-0004, Japan
  3. Institute of Floricultural Science, National Agriculture and Food Research Organization, Fujimoto 2-1, Tsukuba, Ibaraki, 305-8519, Japan
  2. Department of Biotechnology and Life Science, Faculty of Engineering, Tokyo University of Agriculture and Technology, Naka-cho 2-24-16, Koganei, Tokyo, 184-8588, Japan
  
• ISSN：1617-4623

文摘

The molecular mechanisms underlying spontaneous bud mutations, which provide an important breeding tool in carnation, are poorly understood. Here we describe a new active hAT type transposable element, designated Tdic101, the movement of which caused a bud mutation in carnation that led to a change of flower color from purple to deep pink. The color change was attributed to Tdic101 insertion into the second intron of F3′H, the gene for flavonoid 3-hydroxylase responsible for purple pigment production. Regions on the deep pink flowers of the mutant can revert to purple, a visible phenotype of, as we show, excision of the transposable element. Sequence analysis revealed that Tdic101 has the characteristics of an autonomous element encoding a transposase. A related, but non-autonomous element dTdic102 was found to move in the genome of the bud mutant as well. Its mobilization might be the result of transposase activities provided by other elements such as Tdic101. In carnation, therefore, the movement of transposable elements plays an important role in the emergence of a bud mutation.