Methylation status of long interspersed element-1 in advanced gastric cancer and its prognostic implication

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• 作者：Young Seok Song ; Younghoon Kim ; Nam Yun Cho ; Han Kwang Yang ; Woo Ho Kim…
• 关键词：Gastric cancer ; Long interspersed element ; 1 ; Hypomethylation ; Prognosis
• 刊名：Gastric Cancer
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：19
• 期：1
• 页码：98-106
• 全文大小：426 KB
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• 作者单位：Young Seok Song (1)
  Younghoon Kim (2)
  Nam Yun Cho (1)
  Han Kwang Yang (3)
  Woo Ho Kim (2)
  Gyeong Hoon Kang (1) (2)
  
  1. Laboratory of Epigenetics, Cancer Research Institute, Seoul National University College of Medicine, Seoul, Korea
  2. Department of Pathology, Seoul National University College of Medicine, 28 Yongon-dong, Chongno-gu, Seoul, 110-744, South Korea
  3. Department of General Surgery, Seoul National University College of Medicine, Seoul, Korea
  
• 刊物类别：Medicine
• 刊物主题：Medicine & Public Health
  Oncology
  Gastroenterology
  Surgical Oncology
  Pathology
  Radiotherapy
  
• 出版者：Springer Japan
• ISSN：1436-3305

文摘

Backgrounds Reportedly, the pyrosequencing methylation assay can produce inconsistent results between paired snap-frozen and formalin-fixed paraffin-embedded archival tissue samples. In this study, we assayed the methylation levels at four individual CpG sites of L1 using pyrosequencing and found that the methylation levels at individual CpG sites were different but were closely correlated between paired snap-frozen and formalin-fixed paraffin-embedded tissue samples. We aimed to determine whether low methylation status of L1 is associated with gastric cancer patient prognosis.