Comparative studies on manual and automatic backbone chemical shift assignments of 2H/13C/15N-labeled Ube2g1
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- 作者：Yun-Seok Choi ; Eun-Hee Kim ; Kyoung-Seok Ryu
- 关键词：Chemical shift assignment ; Deuteration ; Homologous modeling ; NMR ; RASPnmr ; SHIFTX2 ; Ube2g1
- 刊名：Journal of Analytical Science and Technology
- 出版年：2015
- 出版时间：December 2015
- 年：2015
- 卷：6
- 期：1
- 全文大小：881 KB
- 刊物主题：Analytical Chemistry; Characterization and Evaluation of Materials; Environmental Monitoring/Analysis;
- 出版者：Springer Netherlands
- ISSN：2093-3371
文摘
Background Ube2g1, one of human E2 enzymes, possesses an additional acidic loop in the vicinity of the active Cys90 residue. The presence of the backbone chemical shifts (bCSs) is essential for various NMR studies of Ube2g1. Triple resonance experiments for the bCSs assignment of 13C/15N-labeled Ube2g1 were not efficient due to the relatively high molecular size (more than 20 kDa).