Protective effects of Lycium barbarum polysaccharide on neonatal rat primary cultured hippocampal neurons injured by oxygen-glucose deprivation and reperfusion
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- 作者：Chen Rui (1)
Li Yuxiang (2)
Hao Yinju (1)
Zhu Qingluan (1)
Wu Yang (1)
Zhao Qipeng (1)
Wang Hao (3)
Ma Lin (4)
Liu Juan (5)
Zhao Chengjun (5)
Jiang Yuanxu (1)
Wang Yanrong (6)
Dai Xiuying (7)
Zhang Wannian (8)
Sun Tao (4)
Yu Jianqiang (9) yujq910315@163.com - 关键词：Lycium barbarum polysaccharide (LBP) – ; Hippocampal neurons – ; Oxygen ; glucose deprivation and reperfusion – ; Neuroprotection
- 刊名：Journal of Molecular Histology
- 出版年：2012
- 出版时间：October 2012
- 年：2012
- 卷：43
- 期：5
- 页码：535-542
- 全文大小：546.5 KB
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- ISSN：1567-2387
文摘
This study investigated the protective effects of Lycium barbarum polysaccharide (LBP) on alleviating injury from oxygen-glucose deprivation/reperfusion (OGD/RP) in primary cultured rat hippocampal neurons. Cultured hippocampal neurons were exposed to oxygen-glucose deprivation (OGD) for 2 h followed by a 24 h re-oxygenation. The MTT assay and the lactate dehydrogenase (LDH) release were used to determine the neuron viability. Superoxide dismutase (SOD), Glutathione peroxidase (GSH-PX), malondialdehyde (MDA) were determined by spectrophotometry using commercial kits. Mitochondrial membrane potential (MMP) and the intracellular free calcium concentration ([Ca2+]i) in hippocampal neurons were measured using the confocal laser scanning microscope (CLSM). Treatment with LBP (10–40 mg/l) significantly attenuated neuronal damage and inhibited LDH release in a dose-dependent manner. Furthermore, LBP enhanced activities of SOD and GSH-PX but it decreased their MDA content, inhibited [Ca2+]i elevation and decrease of MMP in ischemia–reperfusion treated hippocampal neurons. These findings suggested that LBP may be a potential neuroprotective agent for cerebral ischemia–reperfusion injury.