Inhibitory effect of chemical combinations on seed germination and pre-harvest sprouting in hybrid rice

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• 作者：Qijuan Hu ; Yuying Fu ; Yajing Guan ; Cheng Lin ; Dongdong Cao…
• 关键词：Pre ; harvest sprouting ; Inhibitor ; Hybrid rice ; Seed ; Germination
• 刊名：Plant Growth Regulation
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：80
• 期：3
• 页码：281-289
• 全文大小：752 KB
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Plant Physiology
  
• 出版者：Springer Netherlands
• ISSN：1573-5087
• 卷排序：80

文摘

Pre-harvest sprouting (PHS) is a primary cause of decreases in quality and economic value in rice seeds. This study examined the inhibitory effects of four combinations of maleic hydrazide, eugenol and uniconazole on rice PHS and seed quality. After the four treatments, delayed grain germination was observed compared with the control, mean germination time was prolonged by 17.5 % and germination index was decreased by an average of 18.2 %. Activity of α-amylase was significantly reduced, by 26.7–83.5 %, during grain germination and early seedling growth. We then used qRT-PCR to further analyze the mRNA expression levels of four genes involved in α-amylase biosynthesis. The expression of OsAMY3B and OsAMY3E was significantly down-regulated by the four combinations of chemicals. In field trials, each of the four combinations resulted in a significantly lower sprouting rate and sprouting index compared with the control. In addition, freshly harvested rice seeds showed no significant differences in the mean germination time or germination index between the four combination treatments and the control. After 1 month of storage, there was still no significant difference between the four combination treatments and the control with regard to the mean germination time and germination index. These results suggest that the selected combinations strongly inhibited the seed germination speed and PHS of rice in the field. However, these combinations had no inhibitory effects on the final germination percentage and showed no negative effect on seed quality after short-term storage. The combinations of inhibitors used in this study can be applied to decrease the PHS damage caused by high temperatures and moist weather during the production of hybrid rice.