Biogas properties and enzymatic analysis during anaerobic fermentation of Phragmites australis straw and cow dung: influence of nickel chloride supplement

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• 作者：Yonglan Tian ; Huayong Zhang ; Yang Chai ; Lijun Wang ; Xueyue Mi ; Luyi Zhang…
• 关键词：Phragmites australis ; Nickel ion ; Enzyme ; Cellulase ; Protease ; Dehydrogenase
• 刊名：Biodegradation
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：28
• 期：1
• 页码：15-25
• 全文大小：
• 刊物类别：Earth and Environmental Science
• 刊物主题：Microbiology; Soil Science & Conservation; Geochemistry; Terrestrial Pollution; Waste Water Technology / Water Pollution Control / Water Management / Aquatic Pollution; Waste Management/Waste Technolo
• 出版者：Springer Netherlands
• ISSN：1572-9729
• 卷排序：28

文摘

The importance of nickel (added as NiCl₂) on mesophilic anaerobic fermentation of Phragmites australis straw and cow dung was demonstrated by investigating the biogas properties, pH values, organic matter degradation [chemical oxygen demand (COD)] and enzyme activities (cellulase, protease and dehydrogenase) during the fermentation process. The results showed that Ni2+ addition increased the cumulative biogas yields by >18 % by improving the efficiency of first peak stage and bringing forward the second peak stage. The pH values were not significantly influenced by Ni2+ addition (p > 0.05). Biogas yields were associated with variations in COD concentrations rather than momentary concentrations. At the start-up stage of fermentation (4th day), the biogas yields increased gradually together with the increase of dehydrogenase activities at elevated Ni2+ concentrations when cellulase and protease activities were similar in all test groups. It is suggested that Ni2+ addition was mainly dependent on the methanogenic stage. After the start-up stage, the impact of Ni2+ addition on biogas production was mainly dependent on its effect on cellulase activities, rather than protease or dehydrogenase activities.