Generation of AQP2-Cre transgenic mini-pigs specifically expressing Cre recombinase in kidney collecting duct cells
详细信息 查看全文
- 作者：Weiwei Luo (1)
Zhanjun Li (1)
Yongye Huang (1)
Yang Han (1)
Chaogang Yao (1)
Xinping Duan (1)
Hongsheng Ouyang (1)
Li Li (1) - 关键词：Aquaporin 2 ; Collecting duct cells ; Cre recombinase ; Kidney ; specific expression ; Mini ; pigs
- 刊名：Transgenic Research
- 出版年：2014
- 出版时间：April 2014
- 年：2014
- 卷：23
- 期：2
- 页码：365-375
- 全文大小：3,715 KB
- 参考文献：1. Ahn D, Ge Y, Stricklett PK, Gill P, Taylor D, Hughes AK, Yanagisawa M, Miller L, Nelson RD, Kohan DE (2004) Collecting duct-specific knockout of endothelin-1 causes hypertension and sodium retention. J Clin Investig 114(4):504-11 CrossRef
2. Bouchard M, Souabni A, Busslinger M (2004) Tissue-specific expression of cre recombinase from the Pax8 locus. Genesis 38(3):105-09 CrossRef
3. Breton S, Alper SL, Gluck SL, Sly WS, Barker JE, Brown D (1995) Depletion of intercalated cells from collecting ducts of carbonic anhydrase II-deficient (CAR2 null) mice. Am J Physiol Renal Physiol 269(6):F761–F774
4. Chang M-Y, Ong AC (2007) Autosomal dominant polycystic kidney disease: recent advances in pathogenesis and treatment. Nephron Physiol 108(1):p1–p7 CrossRef
5. Chen L, Li L, Pang D, Li Z, Wang T, Zhang M, Song N, Yan S, Lai L, Ouyang H (2010) Construction of transgenic swine with induced expression of Cre recombinase. Animal 4(05):767-71 CrossRef
6. Dobie KW, Lee M, Fantes JA, Graham E, Clark AJ, Springbett A, Lathe R, McClenaghan M (1996) Variegated transgene expression in mouse mammary gland is determined by the transgene integration locus. Proc Natl Acad Sci 93(13):6659-664 CrossRef
7. Fedeles SV, Tian X, Gallagher A-R, Mitobe M, Nishio S, Lee SH, Cai Y, Geng L, Crews CM, Somlo S (2011) A genetic interaction network of five genes for human polycystic kidney and liver diseases defines polycystin-1 as the central determinant of cyst formation. Nat Genet 43(7):639-47 CrossRef
8. Festenstein R, Tolaini M, Corbella P, Mamalaki C, Parrington J, Fox M, Miliou A, Jones M, Kioussis D (1996) Locus control region function and heterochromatin-induced position effect variegation. Science 271(5252):1123-125 CrossRef
9. Fu J, Teucher M, Anastassiadis K, Skarnes W, Stewart AF (2010) Chapter eight-A recombineering pipeline to make conditional targeting constructs. Methods Enzymol 477:125-44 CrossRef
10. Fushimi K, Uchida S, Hara Y, Hirata Y, Marumo F, Sasaki S (1993) Cloning and expression of apical membrane water channel of rat kidney collecting tubule. Nature 361(6412):549-52 CrossRef
11. Grantham JJ (2008) Autosomal dominant polycystic kidney disease. N Engl J Med 359(14):1477-485 CrossRef
12. Guan Y, Hao C, Cha DR, Rao R, Lu W, Kohan DE, Magnuson MA, Redha R, Zhang Y, Breyer MD (2005) Thiazolidinediones expand body fluid volume through PPARγ stimulation of ENaC-mediated renal salt absorption. Nat Med 11(8):861-66 CrossRef
13. Harris PC, Torres VE (2009) Polycystic kidney disease. Annu Rev Med 60:321 CrossRef
14. He R-H, Sheng J-Z, Luo Q, Jin F, Wang B, Qian Y-L, Zhou C-Y, Sheng X, Huang H-F (2006) Aquaporin-2 expression in human endometrium correlates with serum ovarian steroid hormones. Life Sci 79(5):423-29 CrossRef
15. Ingham DJ, Beer S, Money S, Hansen G (2001) Quantitative real-time PCR assay for determining transgene copy number in transformed plants. Biotechniques 31(1):132-41
16. Kessenbrock K, Plaks V, Werb Z (2010) Matrix metalloproteinases: regulators of the tumor microenvironment. Cell 141(1):52-7 CrossRef
17. Koetsier PA, Mangel L, Schmitz B, Doerfler W (1996) Stability of transgene methylation patterns in mice: position effects, strain specificity and cellular mosaicism. Transgenic Res 5(4):235-44 CrossRef
18. Kong Q, Wu M, Huan Y, Zhang L, Liu H, Bou G, Luo Y, Mu Y, Liu Z (2009) Transgene expression is associated with copy number and cytomegalovirus promoter methylation in transgenic pigs. PLoS One 4(8):e6679 CrossRef
19. Lai L, Prather RS (2003a) Creating genetically modified pigs by using nuclear transfer. Reprod Biol Endocrinol 1(1):82 CrossRef
20. Lai L, Prather RS (2003b) Production of cloned pigs by using somatic cells as donors. Cloning Stem Cells 5(4):233-41 CrossRef
21. Le Y, Sauer B (2000) Conditional gene knockout using cre recombinase. In: Tuan RS, Lo CW (eds) Developmental biology protocols, vol II. Humana Press Inc., Totowa, pp 477-85
22. Lerman LO, Schwartz RS, Grande JP, Sheedy PF, Romero JC (1999) Noninvasive evaluation of a novel swine model of renal artery stenosis. J Am Soc Nephrol 10(7):1455-465
23. Leuchs S, Saalfrank A, Merkl C, Flisikowska T, Edlinger M, Durkovic M, Rezaei N, Kurome M, Zakhartchenko V, Kessler B (2012) Inactivation and inducible oncogenic mutation of p53 in gene targeted pigs. PLoS One 7(10):e43323 CrossRef
24. Li L, Pang D, Wang T, Li Z, Chen L, Zhang M, Song N, Nie D, Chen Z, Lai L (2009) Production of a reporter transgenic pig for monitoring Cre recombinase activity. Biochem Biophys Res Commun 382(2):232-35 CrossRef
25. Lunney JK (2007) Advances in swine biomedical model genomics. Int J Biol Sci 3(3):179 CrossRef
26. Melo EO, Canavessi AM, Franco MM, Rumpf R (2007) Animal transgenesis: state of the art and applications. J Appl Genet 48(1):47-1 CrossRef
27. Mott JD, Werb Z (2004) Regulation of matrix biology by matrix metalloproteinases. Curr Opin Cell Biol 16(5):558-64 CrossRef
28. Nagy A (2000) Cre recombinase: the universal reagent for genome tailoring. Genesis 26(2):99-09 CrossRef
29. Nakanishi T, Kuroiwa A, Yamada S, Isotani A, Yamashita A, Tairaka A, Hayashi T, Takagi T, Ikawa M, Matsuda Y (2002) FISH analysis of 142 EGFP transgene integration sites into the mouse genome. Genomics 80(6):564-74 CrossRef
30. Nelson RD, Stricklett P, Gustafson C, Stevens A, Ausiello D, Brown D, Kohan DE (1998) Expression of an AQP2 Cre recombinase transgene in kidney and male reproductive system of transgenic mice. Am J Physiol Cell Physiol 275(1):C216–C226
31. Nielsen S, DiGiovanni SR, Christensen EI, Knepper MA, Harris HW (1993) Cellular and subcellular immunolocalization of vasopressin-regulated water channel in rat kidney. Proc Natl Acad Sci 90(24):11663-1667 CrossRef
32. Ong AC, Harris PC (2005) Molecular pathogenesis of ADPKD: the polycystin complex gets complex. Kidney Int 67(4):1234-247 CrossRef
33. Pillai MM, Venkataraman GM, Kosak S, Torok-Storb B (2008) Integration site analysis in transgenic mice by thermal asymmetric interlaced (TAIL)-PCR: segregating multiple-integrant founder lines and determining zygosity. Transgenic Res 17(4):749-54 CrossRef
34. Prather RS, Shen M, Dai Y (2008) Genetically modified pigs for medicine and agriculture. Biotechnol Genet Eng Rev 25(1):245-66
35. Pruijt JF, Fibbe WE, Laterveer L, Pieters RA, Lindley IJ, Paemen L, Masure S, Willemze R, Opdenakker G (1999) Prevention of interleukin-8-induced mobilization of hematopoietic progenitor cells in rhesus monkeys by inhibitory antibodies against the metalloproteinase gelatinase B (MMP-9). Proc Natl Acad Sci 96(19):10863-0868 CrossRef
36. Rahman MA, Hwang G-L, Razak SA, Sohm F, Maclean N (2000) Copy number related transgene expression and mosaic somatic expression in hemizygous and homozygous transgenic tilapia ( / Oreochromis niloticus). Transgenic Res 9(6):417-27 CrossRef
37. Ronzaud C, Loffing J, Bleich M, Gretz N, Gr?ne H-J, Schütz G, Berger S (2007) Impairment of sodium balance in mice deficient in renal principal cell mineralocorticoid receptor. J Am Soc Nephrol 18(6):1679-687 CrossRef
38. Rubera I, Hummler E, Beermann F (2009) Transgenic mice and their impact on kidney research. Pflügers Arch 458(1):211-22 CrossRef
39. Serova IA, Dvoryanchikov GA, Andreeva LE, Burkov IA, Dias LP, Battulin NR, Smirnov AV, Serov OL (2012) A 3,387?bp 5-flanking sequence of the goat alpha-S1-casein gene provides correct tissue-specific expression of human granulocyte colony-stimulating factor (hG-CSF) in the mammary gland of transgenic mice. Transgenic Res 21(3):485-98 CrossRef
40. Skowronski MT, Kwon T-H, Nielsen S (2009) Immunolocalization of aquaporin 1, 5, and 9 in the female pig reproductive system. J Histochem Cytochem 57(1):61-7 CrossRef
41. Srinivas S, Watanabe T, Lin C-S, William CM, Tanabe Y, Jessell TM, Costantini F (2001) Cre reporter strains produced by targeted insertion of EYFP and ECFP into the ROSA26 locus. BMC Dev Biol 1(1):4 CrossRef
42. Walters E, Wolf E, Whyte J, Mao J, Renner S, Nagashima H, Kobayashi E, Zhao J, Wells K, Critser J (2012) Completion of the swine genome will simplify the production of swine as a large animal biomedical model. BMC Med Genomics 5(1):55 CrossRef
43. Whitworth KM, Prather RS (2010) Somatic cell nuclear transfer efficiency: how can it be improved through nuclear remodeling and reprogramming? Mol Reprod Dev 77(12):1001-015 CrossRef
44. Whyte JJ, Prather RS (2011) Genetic modifications of pigs for medicine and agriculture. Mol Reprod Dev 78(10-1):879-91 CrossRef
45. Yang B (2000) The human aquaporin gene family. Curr Genomics 1(1):91-02 CrossRef
46. Ye J, He J, Li Q, Feng Y, Bai X, Chen X, Zhao Y, Hu X, Yu Z, Li N (2013) Generation of c-Myc transgenic pigs for autosomal dominant polycystic kidney disease. Transgenic Res 22(6):1231-239
47. Zhang H, Zhang A, Kohan DE, Nelson RD, Gonzalez FJ, Yang T (2005) Collecting duct-specific deletion of peroxisome proliferator-activated receptor γ blocks thiazolidinedione-induced fluid retention. Proc Natl Acad Sci USA 102(26):9406-411 CrossRef
48. Zhou Z, Ma H, Qu L, Xie F, Ma Q, Ren Z (2012) Establishment of an improved high-efficiency thermal asymmetric interlaced PCR for identification of genomic integration sites mediated by phiC31 integrase. World J Microbiol Biotechnol 28(3):1295-299 CrossRef - 作者单位：Weiwei Luo (1)
Zhanjun Li (1)
Yongye Huang (1)
Yang Han (1)
Chaogang Yao (1)
Xinping Duan (1)
Hongsheng Ouyang (1)
Li Li (1)
1. College of Animal Science, Jilin University, 5333 Xi’an Road, Changchun, 130062, People’s Republic of China - ISSN：1573-9368
文摘
The important differences in physiological parameters and anatomical characteristics of the kidney between humans and mice make it difficult to replicate the precise progression of human renal cystic diseases in gene modification mouse models. In contrast to mice, pigs are a better animal model of human diseases, as they are more similar in terms of organ size, structure, and physiological parameters. Here, we report the generation and initial examination of an AQP2-Cre transgenic (Tg) Chinese miniature (mini)-pig line that expresses Cre recombinase exclusively in kidney collecting duct cells. An 8-kb fragment of the mini-pig aquaporin 2 (AQP2) 5-flanking region was utilized to direct Cre expression in Tg mini-pigs. Two Tg mini-pigs were generated by pig somatic cell nuclear transfer and both carried the entire coding sequence of Cre recombinase. RT-PCR and western blotting analysis revealed that Cre recombinase was uniquely expressed in the kidney, while immunohistochemical studies located its expression in kidney collecting duct cells. Furthermore, six integration sites and 12-4 copies of the Cre gene were detected in various tissues by high-efficiency thermal asymmetric interlaced PCR and absolute quantitative real-time PCR, respectively. Combined with previous studies of Cre recombinase activity, we believe that this AQP2-Cre Tg mini-pig line will be a useful tool to generate kidney collecting duct cell-specific gene knockout mini-pig models, thereby allowing the investigation of gene functions in kidney development and the mechanisms of human renal cystic disease.