Response surface optimization of cholesterol extraction from lanolin alcohol by selective solvent crystallization
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- 作者：Hui Ding ; Dan Zhao ; Yujie Gao
- 关键词：Selective solvent crystallization ; Response surface methodology ; Cholesterol ; Crude lanosterol ; Extraction
- 刊名：Chemical Papers
- 出版年：2017
- 出版时间：January 2017
- 年：2017
- 卷：71
- 期：1
- 页码：71-79
- 全文大小：
- 刊物类别：Chemistry and Materials Science
- 刊物主题：Chemistry/Food Science, general; Industrial Chemistry/Chemical Engineering; Biochemistry, general; Medicinal Chemistry; Materials Science, general; Biotechnology;
- 出版者：Springer International Publishing
- ISSN：1336-9075
- 卷排序：71
文摘
A process to extract cholesterol from lanolin alcohol by selective solvent crystallization and recrystallization was developed. The crude lanosterol crystallization process was performed in methanol-acetone (3:1, v/v) as solvent at 18 mL g−1 liquid–solid ratio and 20.0 °C for 6 h. To be optimized by response surface methodology (RSM) coupled with central composite design, the process of cholesterol crystallization was carried out in acetic acid as solvent at 4.18 mL g−1 liquid–solid ratio and 26.1 °C for 6 h. Under the optimized conditions, cholesterol purity was substantially improved increasing from 17.5 to 76.2%, while the purity and recovery efficiency of cholesterol increased to 94.7 and 88.0% after recrystallization, respectively.