Improved hydrogen photoproduction regulated by carbonylcyanide m-chlorophenylhrazone from marine green alga Platymonas subcordiformis grown in CO₂-supplemented air bubble column bioreactor

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• 作者：Zhen Guo ; Zhaoan Chen ; Wei Zhang ; Xingju Yu and Meifang Jin
• 关键词：Bioreactor ; Carbon dioxide ; Carbonylcyanide m ; chlorophenylhydrazone (CCCP) ; Hydrogen ; Platymonas subcordiformis
• 刊名：Biotechnology Letters
• 出版年：2008
• 出版时间：May, 2008
• 年：2008
• 卷：30
• 期：5
• 页码：877-883
• 全文大小：276.5 KB

文摘

To develop an integrated process of CO₂-fixation and H₂ photoproduction by marine green microalga Platymonas subcordiformis, the impact of algal cells grown in CO₂-supplemented air bubble column bioreactor was investigated on H₂ photoproduction regulated by carbonylcyanide m-chlorophenylhrazone. Highest cell growth (3.85 ¡Á 106 cells ml−1), starch content (0.25 ¡À 0.08 mg per 106 cells) and hydrogen production (50 ¡À 3 ml l−1) were achieved at 3 % CO₂-supplemented culture, which are respectively 1.4, 2.1, 1.5-fold of the air-supplemented culture. Improved H₂ production correlated well with the increase in starch accumulation. In this process, the algal cells have been recycled for stable H₂ production of 40–50 ml l−1 over five cycles.