Restricted-access material HPLC method for simultaneous determination of carboxylate and lactone forms of topotecan in human serum

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• 作者：Jun Ma ; Zheng-Ping Jia ; Zhi-Qiang Guo ; Peng-Cheng Fan and Rong Wang
• 刊名：Journal of Analytical Chemistry
• 出版年：2009
• 出版时间：August, 2009
• 年：2009
• 卷：64
• 期：8
• 页码：823-827
• 全文大小：222.1 KB

文摘

A simple restricted-access media (RAM) HPLC method for simultaneous determination of lactone and carboxylate forms of topotecan (TPT) in human serum was established. Using a RAM analytical column, serum samples were directly injected into the HPLC system. The eluted peaks of two forms of TPT were monitored with a fluorescence detector. The separation was completed in 18 min. The linear range was 15–1000 ng/mL, intra-day and inter-day variations being less than 7 % . The kinetic equation was constructed according to the analytical results. The equation shows that the course of TPT lactone form converting to carboxylate form in human serum at 37¡ãC follows a first-order kinetics. Concentration of each form at the moment of sampling was calculated by extrapolation.