Comparison of the Neural Differentiation Potential of Human Mesenchymal Stem Cells from Amniotic Fluid and Adult Bone Marrow
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- 作者：Zhong-Jie Yan (1) (2)
Yu-Qin Hu (3)
Hong-Tian Zhang (2)
Peng Zhang (2)
Zong-Yu Xiao (1)
Xin-Lin Sun (1)
Ying-Qian Cai (1)
Chang-Chen Hu (1)
Ru-Xiang Xu (2) - 关键词：Amniotic fluid ; Bone marrow ; Mesenchymal stem cells ; Neural differentiation ; Neural stem cells
- 刊名：Cellular and Molecular Neurobiology
- 出版年：2013
- 出版时间：May 2013
- 年：2013
- 卷：33
- 期：4
- 页码：465-475
- 全文大小：977KB
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Yu-Qin Hu (3)
Hong-Tian Zhang (2)
Peng Zhang (2)
Zong-Yu Xiao (1)
Xin-Lin Sun (1)
Ying-Qian Cai (1)
Chang-Chen Hu (1)
Ru-Xiang Xu (2)
1. Neurosurgery Institute, Key Laboratory on Brain Function Repair and Regeneration of Guangdong, Zhujiang Hospital, Southern Medical University, Guangzhou, 510282, China
2. Affiliated Bayi Brain Hospital, The Military General Hospital of Beijing PLA, The Bayi Clinical Medical Institute of Southern Medical University, Beijing, 100700, China
3. Department of Gynecology and Obstetrics, The Affiliated Hexian Memorial Hospital of Southern Medical University, Guangzhou, 511400, China - ISSN：1573-6830
文摘
Human mesenchymal stem cells (MSCs) are considered a promising tool for cell-based therapies of nervous system diseases. Bone marrow (BM) has been the traditional source of MSCs (BM-MSCs). However, there are some limitations for their clinical use, such as the decline in cell number and differentiation potential with age. Recently, amniotic fluid (AF)-derived MSCs (AF-MSCs) have been shown to express embryonic and adult stem cell markers, and can differentiate into cells of all three germ layers. In this study, we isolated AF-MSCs from second-trimester AF by limiting dilution and compared their proliferative capacity, multipotency, neural differentiation ability, and secretion of neurotrophins to those of BM-MSCs. AF-MSCs showed a higher proliferative capacity and more rapidly formed and expanded neurospheres compared to those of BM-MSCs. Both immunocytochemical and quantitative real-time PCR analyses demonstrated that AF-MSCs showed higher expression of neural stemness markers than those of BM-MSCs following neural stem cell (NSC) differentiation. Furthermore, the levels of brain-derived growth factor and nerve growth factor secreted by AF-MSCs in the culture medium were higher than those of BM-MSCs. In addition, AF-MSCs maintained a normal karyotype in long-term cultures after NSC differentiation and were not tumorigenic in vivo. Our findings suggest that AF-MSCs are a promising and safe alternative to BM-MSCs for therapy of nervous system diseases.