BMP-2, VEGF and bFGF synergistically promote the osteogenic differentiation of rat bone marrow-derived mesenchymal stem cells

详细信息    查看全文

• 作者：Yan Bai (1)
  Peipei Li (1)
  Guangfu Yin (1)
  Zhongbing Huang (1)
  Xiaoming Liao (1)
  Xianchun Chen (1)
  Yadong Yao (1)
  
• 关键词：BMP ; 2 ; bFGF ; MSC ; Osteogenic differentiation ; VEGF
• 刊名：Biotechnology Letters
• 出版年：2013
• 出版时间：March 2013
• 年：2013
• 卷：35
• 期：3
• 页码：301-308
• 全文大小：915KB
• 参考文献：1. Ball SG, Shuttleworth CA, Kielty CM (2007) Vascular endothelial growth factor can signal through platelet-derived growth factor receptors. J Cell Biol 177:489-00 CrossRef
  2. Bianco P, Robey PG (2001) Stem cells in tissue engineering. Nature 414:118-21 CrossRef
  3. Chen D, Zhao M, Mundy GR (2004) Bone morphogenetic proteins. Growth Factors 22:233-41 CrossRef
  4. Deschaseaux F, Sensébé L, Heymann D (2009) Mechanisms of bone repair and regeneration. Trends Mol Med 15:417-29 CrossRef
  5. Dudley AT, Godin RE, Robertson EJ (1999) Interaction between FGF and BMP signalling pathways regulates development of metanephric mesenchyme. Genes Dev 13:1601-613 CrossRef
  6. Einhorn TA (2005) The science of fracture healing. J Orthop Trauma 19:S4–S6 CrossRef
  7. Fakhry A, Ratisoontorn C, Vedhachalam C, Salhab I, Koyama E, Leboy P, Pacifici M, Kirschner RE (2005) Effects of FGF-2/-9 in calvarial bone cell cultures: differentiation stage-dependent mitogenic effect, inverse regulation of BMP-2 and noggin, and enhancement of osteogenic potential. Bone 36:254-66 CrossRef
  8. Fei Y, Xiao L, Doetschman T, Coffin DJ, Hurley MM (2011) Fibroblast growth factor 2 stimulation of osteoblast differentiation and bone formation is mediated by modulation of the Wnt signaling pathway. J Biol Chem 286:40575-0583 CrossRef
  9. Huang ZN, Ren PG, Ma T, Smith RL, Goodman SB (2010) Modulating osteogenesis of mesenchymal stem cells by modifying growth factor availability. Biomaterials 51:305-10
  10. Hulliger J (2003) Connective tissue polarity unraveled by a markov-chain mechanism of collagen fibril segment self-assembly. Biophys J 84:3501-507 CrossRef
  11. Kempen DHR, Lu L, Heijink A, Hefferan TE, Creemers LB, Maran A, Yaszemski MJ, Dhert WJA (2009) Effect of local sequential VEGF and BMP-2 delivery on ectopic and orthotopic bone regeneration. Biomaterials 30:2816-825 CrossRef
  12. Kim S, Kang YQ, Krueger CA, Sen M, Holcomb JB, Chen D, Wenke JC, Yang YZ (2012) Sequential delivery of BMP-2 and IGF-1 using a chitosan gel with gelatin microspheres enhance early osteoblastic differentiation. Acta Biomater 5:1768-777 CrossRef
  13. Maegawa N, Kawamura K, Hirose M, Yajima H, Takakura Y, Ohgushi H (2007) Enhancement of osteoblastic differentiation of mesenchymal stromal cells cultured by selective combination of bone morphogenetic protein-2 (BMP-2) and fibroblast growth factor-2 (FGF-2). J Tissue Eng Regen Med 1:306-13 CrossRef
  14. Miyazono K, Maeda S, Imamura T (2005) BMP receptor signaling: transcriptional targets regulation of signals, and signaling cross-talk. Cytokine Growth Factor Rev 16:251-63 CrossRef
  15. Orlandini M, Spreafico A, Bardelli M, Rocchigiani M, Salameh A, Nucciotti S, Capperucci C, Frediani B, Oliviero S (2006) Vascular endothelial growth factor-D activates VEGFR-3 expressed in osteoblasts inducing their differentiation. J Biol Chem 281:17961-7967 CrossRef
  16. Peng H, Usas A, Olshanski A, Ho AM, Gearhart B, Cooper GM, Huard J (2005) VEGF improves, whereas sFlt1 inhibits, BMP2-induced bone formation and bone healing through modulation of angiogenesis. J Bone Miner Res 20:2017-027 CrossRef
  17. Petite H, Viateau V, Bensaid W, Meunier A, Pollak C, Bourguignon M, Oudina K, Sedel L, Guillemin G (2000) Tissue-engineered bone regeneration. Nat Biotechnol 18:959-63 CrossRef
  18. Pittenger MF, Mackay AM, Beck SC, Jaiswal RK, Douglas R, Mosca JD, Moorman MA, Simonetti DW, Craig S, Marshak DR (1999) Multilineage potential of adult human mesenchymal stem cells. Science 284:143-47 CrossRef
  19. Ryoo HM, Lee MH, Kim YJ (2006) Critical molecular switches involved in BMP-2-induced osteogenic differentiation of mesenchymal cells. Gene 366:51-7 CrossRef
  20. Song I, Kim BS, Kim CS, Ima GI (2011) Effects of BMP-2 and vitamin D3 on the osteogenic differentiation of adipose stem cells. Biochem Biophys Res Commun 408:126-31 CrossRef
  
• 作者单位：Yan Bai (1)
  Peipei Li (1)
  Guangfu Yin (1)
  Zhongbing Huang (1)
  Xiaoming Liao (1)
  Xianchun Chen (1)
  Yadong Yao (1)
  
  1. College of Materials Science and Engineering, Sichuan University, Chengdu, 610064, People’s Republic of China
  
• ISSN：1573-6776

文摘

Mesenchymal stem cells (MSCs) were treated with bone morphogenetic protein-2 (BMP-2), vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) dose-dependently and time-dependently. Together they caused a strong synergistic effect on the osteogenic differentiation of MSCs, with lower concentrations of each factor being enough to show the synergistic promotion (50?ng BMP-2/ml, 1?ng VEGF/ml and 10?ng bFGF/ml). When both VEGF and bFGF were added in the early proliferating stage (the first 7?days) and BMP-2 was added in the late differentiation stage (the last 7?days), osteogenic differentiation of MSCs could be enhanced more effectively.