A broadly reactive monoclonal antibody detects multiple genotypes of hepatitis B virus X protein

详细信息    查看全文

• 作者：Lili Wei (1)
  Zhongliang Shen (1)
  Xue Zhao (1)
  Yanxin Wu (1)
  Wei Liu (1)
  Junqi Zhang (1)
  Youhua Xie (1)
  Jing Liu (1)
  
• 关键词：HBV ; Genotype ; HBx ; Protein expression
• 刊名：Archives of Virology
• 出版年：2014
• 出版时间：October 2014
• 年：2014
• 卷：159
• 期：10
• 页码：2731-2735
• 全文大小：925 KB
• 参考文献：1. Cotelesage JJ, Osiowy C, Lawrence C, DeVarennes SL, Teow S, Beniac DR, Booth TF (2011) Hepatitis B virus genotype G forms core-like particles with unique structural properties. J Viral Hepat 18:443鈥?48 CrossRef
  2. Guerrieri F, Belloni L, Pediconi N, Levrero M (2013) Molecular mechanisms of HBV-associated hepatocarcinogenesis. Semin Liver Dis 33:147鈥?56 CrossRef
  3. Hu Z, Zhang Z, Doo E, Coux O, Goldberg AL, Liang TJ (1999) Hepatitis B virus X protein is both a substrate and a potential inhibitor of the proteasome complex. J Virol 73:7231鈥?240
  4. Ladner SK, Otto MJ, Barker CS, Zaifert K, Wang GH, Guo JT, Seeger C, King RW (1997) Inducible expression of human hepatitis B virus (HBV) in stably transfected hepatoblastoma cells: a novel system for screening potential inhibitors of HBV replication. Antimicrob Agents Chemother 41:1715鈥?720
  5. Li K, Zoulim F, Pichoud C, Kwei K, Villet S, Wands J, Li J, Tong S (2007) Critical role of the 36-nucleotide insertion in hepatitis B virus genotype G in core protein expression, genome replication, and virion secretion. J Virol 81:9202鈥?215 CrossRef
  6. Liang TJ (2009) Hepatitis B: the virus and disease. Hepatology 49:S13鈥揝21 CrossRef
  7. Lucifora J, Arzberger S, Durantel D, Belloni L, Strubin M, Levrero M, Zoulim F, Hantz O, Protzer U (2011) Hepatitis B virus X protein is essential to initiate and maintain virus replication after infection. J Hepatol 55:996鈥?003 CrossRef
  8. Murakami S (2001) Hepatitis B virus X protein: a multifunctional viral regulator. J Gastroenterol 36:651鈥?60 CrossRef
  9. Osiowy C, Gordon D, Borlang J, Giles E, Villeneuve JP (2008) Hepatitis B virus genotype G epidemiology and co-infection with genotype A in Canada. J Gen Virol 89:3009鈥?015 CrossRef
  10. Pujol FH, Navas MC, Hainaut P, Chemin I (2009) Worldwide genetic diversity of HBV genotypes and risk of hepatocellular carcinoma. Cancer Lett 286:80鈥?8 CrossRef
  11. Schaefer S (2005) Hepatitis B virus: significance of genotypes. J Viral Hepat 12:111鈥?24 CrossRef
  12. Schaefer S (2007) Hepatitis B virus taxonomy and hepatitis B virus genotypes. World J Gastroenterol 13:14鈥?1 CrossRef
  13. Seeger C, Mason WS (2000) Hepatitis B virus biology. Microbiol Mol Biol Rev 64:51鈥?8 CrossRef
  14. Sells MA, Chen ML, Acs G (1987) Production of hepatitis B virus particles in Hep G2 cells transfected with cloned hepatitis B virus DNA. Proc Natl Acad Sci USA 84:1005鈥?009 CrossRef
  15. Stuyver L, De Gendt S, Van Geyt C, Zoulim F, Fried M, Schinazi RF, Rossau R (2000) A new genotype of hepatitis B virus: complete genome and phylogenetic relatedness. J Gen Virol 81:67鈥?4
  16. Tsuge M, Hiraga N, Akiyama R, Tanaka S, Matsushita M, Mitsui F, Abe H, Kitamura S, Hatakeyama T, Kimura T, Miki D, Mori N, Imamura M, Takahashi S, Hayes CN, Chayama K (2010) HBx protein is indispensable for development of viraemia in human hepatocyte chimeric mice. J Gen Virol 91:1854鈥?864 CrossRef
  17. Yu X, Mertz JE (2003) Distinct modes of regulation of transcription of hepatitis B virus by the nuclear receptors HNF4alpha and COUP-TF1. J Virol 77:2489鈥?499 CrossRef
  18. Zoulim F, Locarnini S (2009) Hepatitis B virus resistance to nucleos(t)ide analogues. Gastroenterology 137(1593鈥?608):e1591鈥揺1592
  
• 作者单位：Lili Wei (1)
  Zhongliang Shen (1)
  Xue Zhao (1)
  Yanxin Wu (1)
  Wei Liu (1)
  Junqi Zhang (1)
  Youhua Xie (1)
  Jing Liu (1)
  
  1. Key Laboratory of Medical Molecular Virology (MOE/MOH) and Institutes of Biomedical Sciences, Shanghai Medical College, Fudan University, Shanghai, 200032, People鈥檚 Republic of China
  
• ISSN：1432-8798

文摘

A highly specific and broadly reactive monoclonal antibody against hepatitis B virus X (HBx) protein was developed that detected, in both immunoblotting and immunofluorescence, HBx proteins of seven of the eight currently known genotypes of HBV, which were overexpressed in cultured cells. Evaluation of HBx expression levels in cultured hepatocytes using this monoclonal antibody showed that cells transiently and stably transfected with HBV genomes expressed far less HBx protein than cells transiently transfected with an HBx overexpression plasmid routinely used for studying HBx functions. The availability of such sensitive and broadly reactive monoclonal antibodies against HBx will enable more-quantitative studies of HBx functions.