MoMyb1 is required for asexual development and tissue-specific infection in the rice blast fungus Magnaporthe oryzae
详细信息 查看全文
- 作者：Yanhan Dong ; Qian Zhao ; Xinyu Liu ; Xiaofang Zhang ; Zhongqiang Qi…
- 关键词：Magnaporthe oryzae ; Conidiogenesis ; Stress response ; Cell wall integrity ; Pathogenesis
- 刊名：BMC Microbiology
- 出版年：2015
- 出版时间：December 2015
- 年：2015
- 卷：15
- 期：1
- 全文大小：3,251 KB
- 参考文献：1. Valent, B, Chumley, FG (1991) Molecular genetic analysis of the rice blast fungus Magnaporthe grisea. Annu Rev Phytopathol 29: pp. 443-67 CrossRef
2. Talbot, NJ (2003) On the trail of a cereal killer: Exploring the biology of Magnaporthe grisea. Annu Rev Microbiol 57: pp. 177-202 CrossRef
3. Howard, RJ, Ferrari, MA, Roach, DH, Money, NP (1991) Penetration of hard substrates by a fungus employing enormous turgor pressures. Proc Natl Acad Sci USA 88: pp. 11281-4 CrossRef
4. Jong, JC, McCormack, BJ, Smirnoff, N, Talbot, NJ (1997) Glycerol generates turgor in rice blast. Nature 389: pp. 244-5 CrossRef
5. Pabo, CO, Sauer, RT (1992) Transcription factors: structural families and principles of DNA recognition. Annu Rev Biochem 61: pp. 1053-95 CrossRef
6. Li, G, Zhou, X, Xu, JR (2012) Genetic control of infection-related development in Magnaporthe oryzae. Curr Opin Microbiol 15: pp. 678-84 CrossRef
7. Kim, S, Park, S-Y, Kim, KS, Rho, H-S, Chi, M-H, Choi, J (2009) Homeobox transcription factors are required for conidiation and appressorium development in the rice blast fungus Magnaporthe oryzae. PLoS Genet 5: pp. e1000757 CrossRef
8. Zhang, H, Zhao, Q, Liu, K, Zhang, Z, Wang, Y, Zheng, X (2009) MgCRZ1, a transcription factor of Magnaporthe grisea, controls growth, development and is involved in full virulence. FEMS Microbiol Lett 293: pp. 160-9 CrossRef
9. Guo, M, Chen, Y, Du, Y, Dong, Y, Guo, W, Zhai, S (2011) The bZIP transcription factor MoAP1 mediates the oxidative stress response and is critical for pathogenicity of the rice blast fungus Magnaporthe oryzae. PLoS Pathog 7: pp. e1001302 CrossRef
10. Qi, Z, Wang, Q, Dou, X, Wang, W, Zhao, Q, Lv, R (2012) MoSwi6, an APSES family transcription factor, interacts with MoMps1 and is required for hyphal and conidial morphogenesis, appressorial function and pathogenicity of Magnaporthe oryzae. Mol Plant Pathol 13: pp. 677-89 CrossRef
11. Zhang, H, Zhao, Q, Guo, X, Guo, M, Qi, Z, Tang, W (2014) Pleiotropic function of the putative zinc-finger protein MoMsn2 in Magnaporthe oryzae. Mol Plant Microbe Interact 27: pp. 446-60 CrossRef
12. Guo, M, Guo, W, Chen, Y, Dong, S, Zhang, X, Zhang, H (2010) The basic leucine zipper transcription factor Moatf1 mediates oxidative stress responses and is necessary for full virulence of the rice blast fungus Magnaporthe oryzae. Mol Plant Microbe Interact 23: pp. 1053-68 CrossRef
13. Tang W, Ru Y, Hong L, Zhu Q, Zuo R, Guo X, et al. System-wide characterization of bZIP transcription factor proteins involved in infection-related morphogenesis of Magnaporthe oryzae. Environ Microbiol 2014. doi:10.1111/1462-2920.12618
14. Stracke, R, Werber, M, Weisshaar, B (2001) The R2R3-MYB gene family in Arabidopsis thaliana. Curr Opin Plant Biol 4: pp. 447-56 CrossRef
15. Klempnauer, KH, Gonda, TJ, Bishop, JM (1982) Nucleotide sequence of the retroviral leukemia gene v-myb and its cellular progenitor c-myb: the architecture of a transduced oncogene. Cell 31: pp. 453-63 CrossRef
16. Jin, H, Martin, C (1999) Multifunctionality and diversity within the plant MYB-gene family. Plant Mol Biol 41: pp. 577-85
文摘
Background The Myb super-family of proteins contain a group of functionally diverse transcriptional activators found in plant, animal and fungus. Myb proteins are involved in cell proliferation, differentiation and apoptosis, and have crucial roles in telomeres. The purpose of this study was to characterize the biological function of Myb1 protein in the rice blast fungus Magnaporthe oryzae. Results We identified the Saccharomyces cerevisiae BAS1 homolog MYB1 in M. oryzae, named MoMyb1. MoMyb1 encodes a protein of 322 amino acids and has two SANT domains and is well conserved in various organisms. Targeted gene deletion of MoMYB1 resulted in a significant reduction in vegetative growth and showed defects in conidiation and conidiophore development. Quantitative RT-PCR analysis revealed that the transcription levels of several conidiophore-related genes were apparently decreased in the ΔMomyb1 mutant. Inoculation with mycelia mats displayed that the virulence of the ΔMomyb1 mutant was not changed on rice leaves but was non-pathogenic on rice roots in comparison to the wild type Guy11. In addition, ?em class="a-plus-plus">Momyb1 mutants showed increased resistance to osmotic stresses but more sensitive to cell wall stressor calcofluor white (CFW). Further analysis revealed that MoMyb1 has an important role in the cell wall biosynthesis pathway. Conclusion This study provides the evidence that MoMyb1 is a key regulator involved in conidiogenesis, stress response, cell wall integrity and pathogenesis on rice roots in the filamentous phytopathogen M. oryzae.