Synthesis, characterization, DNA interaction, and antitumor activities of mixed-ligand metal complexes of kaempferol and 1,10-phenanthroline/2,2-bipyridine

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• 作者：Qin Wang (1)
  Min Huang (2)
  Yu Huang (2)
  Jin-Sheng Zhang (3)
  Gui-Feng Zhou (1)
  Ren-Quan Zeng (1)
  Xin-Bin Yang (1)
  
• 关键词：Kaempferol ; Mixed ; ligand metal complexes ; DNA interaction ; Antitumor activity
• 刊名：Medicinal Chemistry Research
• 出版年：2014
• 出版时间：May 2014
• 年：2014
• 卷：23
• 期：5
• 页码：2659-2666
• 全文大小：1,232 KB
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• 作者单位：Qin Wang (1)
  Min Huang (2)
  Yu Huang (2)
  Jin-Sheng Zhang (3)
  Gui-Feng Zhou (1)
  Ren-Quan Zeng (1)
  Xin-Bin Yang (1)
  
  1. Rongchang Campus, Southwest University, Chongqing, 402460, People’s Republic of China
  2. Pharmacy College, Ningxia Medical University, Yinchuan, 750004, People’s Republic of China
  3. School of Chemistry and Material Science, Guizhou Normal University, Guiyang, 550001, People’s Republic of China
  
• ISSN：1554-8120

文摘

Four novel mixed-ligand metal complexes with the composition [Cu(Kae)(phen)]Cl·2H2O (1), [Cu(Kae)(bpy)]Cl·H2O (2), [Zn(Kae)(phen)]Cl·H2O (3), and [Zn(Kae)(bpy)]Cl·H2O (4), where Kae?=?kaempferol, phen?=?1,10-phenanthroline, bpy?=?2,2-bipyridine were synthesized and characterized by elemental analysis, IR, UV, 1H NMR, 13C NMR, HRMS ,and molar conductivity. The binding and cleavage abilities of complexes 1-strong class="a-plus-plus">4 with DNA have been studied by fluorescence spectroscopy, viscosity measurements, and gel electrophoresis under physiological conditions. The experimental results indicated that four complexes could bind to CT-DNA via an intercalative mode, and the complex 1 showed the strongest activity to cleavage DNA (pUC 19). The in vitro cytotoxicities of kaempferol and complexes 1-strong class="a-plus-plus">4 were also evaluated against human breast carcinoma cells by MTT assays. The results show that the cytotoxicities of complexes 1-strong class="a-plus-plus">4 are much higher than that of kaempferol alone, and the complex 1 shows the strongest ability to inhibit the growth of human breast carcinoma cells. The results suggest that the complex 1 may be a valuable tool in cancer chemotherapy agents.