Response of soil bacterial communities to lead and zinc pollution revealed by Illumina MiSeq sequencing investigation
详细信息 查看全文
- 作者：Xihui Xu ; Zhou Zhang ; Shunli Hu ; Zhepu Ruan…
- 关键词：Bacterial community ; Bacterial richness and diversity ; Heavy metals ; Lead and zinc mine ; MiSeq sequencing ; Soil pollution
- 刊名：Environmental Science and Pollution Research
- 出版年：2017
- 出版时间：January 2017
- 年：2017
- 卷：24
- 期：1
- 页码：666-675
- 全文大小：
- 刊物类别：Earth and Environmental Science
- 刊物主题：Environment, general; Environmental Chemistry; Ecotoxicology; Environmental Health; Atmospheric Protection/Air Quality Control/Air Pollution; Waste Water Technology / Water Pollution Control / Water M
- 出版者：Springer Berlin Heidelberg
- ISSN：1614-7499
- 卷排序：24
文摘
Soil provides a critical environment for microbial community development. However, microorganisms may be sensitive to substances such as heavy metals (HMs), which are common soil contaminants. This study investigated bacterial communities using 16S ribosomal RNA (rRNA) gene fragment sequencing in geographic regions with and without HM pollution to elucidate the effects of soil properties and HMs on bacterial communities. No obvious changes in the richness or diversity of bacterial communities were observed between samples from mining and control areas. Significant differences in bacterial richness and diversity were detected between samples from different geographic regions, indicating that the basic soil characteristics were the most important factors affecting bacterial communities other than HMs. However, the abundances of several phyla and genera differed significantly between mining and control samples, suggesting that Zn and Pb pollution may impact the soil bacterial community composition. Moreover, regression analyses showed that the relative abundances of these phyla and genera were correlated significantly with the soil-available Zn and Pb contents. Redundancy analysis indicated that the soil K, ammoniacal nitrogen (NH4+–N), total Cu, and available Zn and Cu contents were the most important factors. Our results not only suggested that the soil bacteria were sensitive to HM stresses but also indicated that other soil properties may affect soil microorganisms to a greater extent.