Hemin-mediated alleviation of zinc, lead and chromium toxicity is associated with elevated photosynthesis, antioxidative capacity; suppressed metal uptake and oxidative stress in rice seedlings

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• 作者：Qin Chen ; Xiaoyan Zhang ; Yuanyuan Liu ; Junyu Wei ; Wenbiao Shen…
• 关键词：Antioxidants ; Antioxidative enzymes ; Hemin ; Heavy ; metal concentrations ; Physiological characteristics ; Rice seedlings
• 刊名：Plant Growth Regulation
• 出版年：2017
• 出版时间：March 2017
• 年：2017
• 卷：81
• 期：2
• 页码：253-264
• 全文大小：
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Plant Sciences; Plant Anatomy/Development; Plant Physiology; Agriculture;
• 出版者：Springer Netherlands
• ISSN：1573-5087
• 卷排序：81

文摘

In this study, we investigated the possible role of hemin in alleviating zinc (Zn), lead (Pb) and chromium (Cr) toxicity in rice seedlings grown hydroponically by analyzing the morphological and physiological parameters. Our results showed that exposure of rice seedlings to excess Zn, Pb or Cr could cause severe leaf chlorosis, inhibit photosynthetic activity and consequently suppress plant growth. The concentration of O₂•− and H₂O₂ significantly increased and the activities of antioxidative enzymes decreased in roots of rice seedlings under metal exposure. The combined treatments (hemin + ZnSO₄, hemin + Pb(NO₃)₂ and hemin + K₂Cr₂O₇), on the other hand, significantly enhanced the photosynthesis- and plant growth-related parameters compared with their corresponding heavy-metal-stress alone. Combined treatments dramatically stimulated the activities of superoxide dismutase (SOD), ascorbic peroxidase (APX) and glutathione reductase (GR) as well as the concentrations of ascorbic acid (AsA) and glutathione (GSH) as compared with the metal- stress alone. The concentrations of reactive oxygen species (ROS, e.g. O₂•− and H₂O₂) were significantly reduced in the metal plus hemin treatments. Hemin addition also reduced metal accumulation in rice seedlings especially in root tissues. These findings suggest that hemin-elevated levels of antioxidants, activities of antioxidative enzymes and hemin-reduced accumulation of heavy-metal could confer resistance against Zn, Pb, and Cr stress in rice seedlings, resulting in improved pigments accumulation, photosynthetic attributes and plant growth.