Calbindin-D9k (CaBP9k) localization and levels of expression in trophoblast cells from human term placenta

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• 作者：Louiza Belkacemi ; Gilles Gariépy ; Catherine Mounier ; Lucie Simoneau and Julie Lafond
• 关键词：Placenta ; CaBP9k ; Cytotrophoblast cells ; Syncytiotrophoblasts ; Human
• 刊名：Cell and Tissue Research
• 出版年：2004
• 出版时间：January 2004
• 年：2004
• 卷：315
• 期：1
• 页码：107-117
• 全文大小：511 KB
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Human Genetics
  Proteomics
  Molecular Medicine
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-0878

文摘

During pregnancy, the calcium (Ca2+) transport machinery of the placenta is solely responsible for the nutrient supply to the developing fetus, where active Ca2+ transport occurs from the mother to the fetus. As part of a larger study to determine the role of Ca2+ in placental transport in vivo, we questioned whether calbindin-D9k (CaBP9k), which is mainly expressed in duodenum, uterus, and placenta of several mammals, is present in cytotrophoblast cells and syncytiotrophoblasts of human term placenta. We were interested in this protein because of its potential importance in serving as an indicator of Ca2+ availability and utilization in the placenta. Here, we demonstrated that CaBP9k transcript is present in both cell types, with a lower expression in cytotrophoblast cells as compared to syncytiotrophoblasts. Moreover, we showed by immunochemistry that CaBP9k protein was present in cytotrophoblast and syncytiotrophoblast placental tissue sections as well as in cultured cells. The occurrence of CaBP9k protein in trophoblast cells was further confirmed by Western blot analysis. Thus, these results indicate for the first time that CaBP9k is unequivocally expressed by trophoblast cells from human term placenta.