Immunomethylomic approach to explore the blood neutrophil lymphocyte ratio (NLR) in glioma survival
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- 作者：John K. Wiencke ; Devin C. Koestler ; Lucas A. Salas…
- 关键词：Glioma ; DNA methylation ; Neutrophil lymphocyte ratio ; Systemic inflammation ; Immunomethylomics
- 刊名：Clinical Epigenetics
- 出版年：2017
- 出版时间：December 2017
- 年：2017
- 卷：9
- 期：1
- 全文大小：1104KB
- 刊物主题：Human Genetics; Gene Function;
- 出版者：BioMed Central
- ISSN：1868-7083
- 卷排序：9
文摘
BackgroundDifferentially methylated regions (DMRs) within DNA isolated from whole blood can be used to estimate the proportions of circulating leukocyte subtypes. We use the term “immunomethylomics” to describe the application of these immune lineage DMRs to studying leukocyte profiles. Here, we applied this approach to peripheral blood DNA from 72 glioma patients with molecularly defined brain tumors, representing common patient groups with defined characteristic survival times and risk factors. We first estimated the proportions of leukocyte subtypes in samples using deconvolution algorithms with reference DMR libraries from isolated leukocyte populations and Illumina 450K DNA methylation data. Then, we calculated the neutrophil to lymphocyte ratio (NLR) using methylation-derived cell composition estimates (mdNLR). The NLR is considered an indicator of immunosuppressive cells in cancer patients.