Gene expression profiles in liver of pigs with extreme high and low levels of androstenone
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- 作者：Maren Moe (1) (2)
Sigbj?rn Lien (2) (3)
Christian Bendixen (4)
Jakob Hedegaard (4)
Henrik Hornsh?j (4)
Ingunn Berget (3) (5)
Theo HE Meuwissen (2) (3)
Eli Grindflek (1) - 刊名：BMC Veterinary Research
- 出版年：2008
- 出版时间：December 2008
- 年：2008
- 卷：4
- 期：1
- 全文大小：644KB
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76. [http://itmat.upenn.edu/~tsprice/software.html] 2007. - 作者单位：Maren Moe (1) (2)
Sigbj?rn Lien (2) (3)
Christian Bendixen (4)
Jakob Hedegaard (4)
Henrik Hornsh?j (4)
Ingunn Berget (3) (5)
Theo HE Meuwissen (2) (3)
Eli Grindflek (1)
1. The Norwegian Pig Breeders Association (NORSVIN), Hamar, Norway
2. Department of Animal and Aquacultural Sciences, Norwegian University of Life Sciences, ?s, Norway
3. Centre for Integrative Genetics (CIGENE), Norwegian University of Life Sciences, ?s, Norway
4. Faculty of Agricultural Sciences, University of Aarhus, Tjele, Denmark
5. Nofima Food, Oslovn 1, ?s, Norway
文摘
Background Boar taint is the unpleasant odour and flavour of the meat of uncastrated male pigs that is primarily caused by high levels of androstenone and skatole in adipose tissue. Androstenone is a steroid and its levels are mainly genetically determined. Studies on androstenone metabolism have, however, focused on a limited number of genes. Identification of additional genes influencing levels of androstenone may facilitate implementation of marker assisted breeding practices. In this study, microarrays were used to identify differentially expressed genes and pathways related to androstenone metabolism in the liver from boars with extreme levels of androstenone in adipose tissue. Results Liver tissue samples from 58 boars of the two breeds Duroc and Norwegian Landrace, 29 with extreme high and 29 with extreme low levels of androstenone, were selected from more than 2500 individuals. The samples were hybridised to porcine cDNA microarrays and the 1% most significant differentially expressed genes were considered significant. Among the differentially expressed genes were metabolic phase I related genes belonging to the cytochrome P450 family and the flavin-containing monooxygenase FMO1. Additionally, phase II conjugation genes including UDP-glucuronosyltransferases UGT1A5, UGT2A1 and UGT2B15, sulfotransferase STE, N-acetyltransferase NAT12 and glutathione S-transferase were identified. Phase I and phase II metabolic reactions increase the water solubility of steroids and play a key role in their elimination. Differential expression was also found for genes encoding 17beta-hydroxysteroid dehydrogenases (HSD17B2, HSD17B4, HSD17B11 and HSD17B13) and plasma proteins alpha-1-acid glycoprotein (AGP) and orosomucoid (ORM1). 17beta-hydroxysteroid dehydrogenases and plasma proteins regulate the availability of steroids by controlling the amount of active steroids accessible to receptors and available for metabolism. Differences in the expression of FMO1, NAT12, HSD17B2 and HSD17B13 were verified by quantitative real competitive PCR. Conclusion A number of genes and pathways related to metabolism of androstenone in liver were identified, including new candidate genes involved in phase I oxidation metabolism, phase II conjugation metabolism, and regulation of steroid availability. The study is a first step towards a deeper understanding of enzymes and regulators involved in pathways of androstenone metabolism and may ultimately lead to the discovery of markers to reduce boar taint.