Specificity of Hydrolysable Tannins from Rhus typhina L. to Oxidants in Cell and Cell-Free Models

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• 作者：Ewa Olchowik-Grabarek ; Saidmukhtar Mavlyanov…
• 关键词：Tannins ; Reactive oxygen and nitrogen species ; Erythrocytes ; tBuOOH ; ONOO− ; HClO
• 刊名：Applied Biochemistry and Biotechnology
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：181
• 期：2
• 页码：495-510
• 全文大小：
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Biotechnology; Biochemistry, general;
• 出版者：Springer US
• ISSN：1559-0291
• 卷排序：181

文摘

Polyphenols of plant origin with wide range of antiradical activity can prevent diseases caused by oxidative and inflammatory processes. In this study, we show using ESR method that the purified water-soluble extract from leaves of Rhus typhina L. containing hydrolysable tannins and its main component, 3,6-bis-O-di-O-galloyl-1,2,4-tri-O-galloyl-β-d-glucose (C₅₅H₄₀O₃₄), displayed a strong antiradical activity against the synthetic 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) in homogenous (solution) and heterogeneous systems (suspension of DPPH containing liposomes) in the range of 1–10 μg/ml. The C₅₅H₄₀O₃₄ and extract at 1–30 μg/ml also efficiently, but to a various degree, decreased reactive oxygen and nitrogen species (RONS) formation induced in erythrocytes by oxidants, following the sequence: tert-butyl hydroperoxide (tBuOOH) > peroxynitrite (ONOO−) >hypochlorous acid (HClO). The explanation of these differences should be seen in the specificity of scavenging different RONS types. These relationships can be represented for C₅₅H₄₀O₃₄ and the extract by the following order of selectivity: O.−₂ ≥ NO· > ·OH > 1O₂. The extract exerted a more pronounced antiradical effect in reaction with DPPH and ROS in all models of oxidative stress in erythrocytes in comparison with C₅₅H₄₀O₃₄. The redox processes between the extract components and their specificity in relation to RONS can underlie this effect.