Time-resolved infrared spectroscopy in the study of photosynthetic systems
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- 作者：Alberto Mezzetti ; Winfried Leibl
- 关键词：FTIR difference spectroscopy ; Step ; scan FTIR ; Vibrational spectroscopy ; Rapid ; scan FTIR ; Infrared ; Electron transfer ; Proton transfer ; Reaction centers ; Ubiquinone ; Light ; harvesting systems ; Bacterial reaction centers ; Photosystem I ; Photosystem II
- 刊名：Photosynthesis Research
- 出版年：2017
- 出版时间：February 2017
- 年：2017
- 卷：131
- 期：2
- 页码：121-144
- 全文大小：
- 刊物类别：Biomedical and Life Sciences
- 刊物主题：Plant Sciences; Plant Physiology; Biochemistry, general; Plant Genetics & Genomics;
- 出版者：Springer Netherlands
- ISSN：1573-5079
- 卷排序：131
文摘
Time-resolved (TR) infrared (IR) spectroscopy in the nanosecond to second timescale has been extensively used, in the last 30 years, in the study of photosynthetic systems. Interesting results have also been obtained at lower time resolution (minutes or even hours). In this review, we first describe the used techniques—dispersive IR, laser diode IR, rapid-scan Fourier transform (FT)IR, step-scan FTIR—underlying the advantages and disadvantages of each of them. Then, the main TR-IR results obtained so far in the investigation of photosynthetic reactions (in reaction centers, in light-harvesting systems, but also in entire membranes or even in living organisms) are presented. Finally, after the general conclusions, the perspectives in the field of TR-IR applied to photosynthesis are described.