A high resolution biostratigraphic study of calcareous nannofossil assemblages has been performed on the Alamedilla section in southern Spain across the Paleocene/Eocene transition. The identification of numerous and well differentiated events together with a major calcareous nannofossil turnover suggest the Alamedilla section to represent one of the most expanded and continuous sections straddling the P/E boundary. Quantitative analyses have revealed a change in the species richness and fluctuations in abundance of important paleoecological species.