γ干扰素诱导蛋白-10对舌鳞状细胞癌细胞CAL-27侵袭性的影响

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英文篇名：The invasion effect of interferon induced protein-10 on human tongue squamous carcinoma cell CAL-27 作者：张兆强 ; 王治平 ; 贾搏 ; 孙翔 ; 陈军 ; 褚洪星 英文作者：ZHANG Zhao-qiang;WANG Zhi-ping;JIA Bo;SUN Xiang;CHEN Jun;CHU Hong-xing;Department of Oral and Maxillofacial Surgery,Stomatological Hospital,Southern Medical University;Department of Oral and Maxillofacial Surgery,Stomatological Hospital in Panyu District,Southern Medical University;Department of Periodontics and Implantology,Stomatological Hospital,Southern Medical University; 关键词：舌鳞状细胞癌 ; 趋化因子受体3 ; γ干扰素诱导蛋白-10 ; 肿瘤侵袭 英文关键词：Tongue squamous cell carcinoma;;CXC-chemokine receptor 3;;Interferon γ-inducible protein-10;;Cell invasion 中文刊名：ZGYI 英文刊名：Chinese Journal of Medicine 机构：南方医科大学口腔医院口腔颌面外科;南方医科大学口腔医院番禺分院口腔颌面外科;南方医科大学口腔医院牙周种植科; 出版日期：2019-07-01 出版单位：中国医刊 年：2019 期：v.37 基金：广东省自然科学基金项目(2018A030313759);; 广东省医学科研基金项目(A2018358);; 广东省省级科技计划项目(2014A020212549);; 南方医科大学科研启动计划-青年培育项目(PY2017N036) 语种：中文; 页：ZGYI201907022 页数：3 CN：07 ISSN：11-3942/R 分类号：73-75

摘要

目的探讨γ干扰素诱导蛋白-10(interferonγ-inducible protein-10,IP-10)对舌鳞状细胞癌细胞侵袭性的影响。方法根据是否加入IP-10将舌鳞状细胞癌细胞株CAL-27分为IP-10组(80 000pg/ml)和对照组两组,采用Transwell侵袭实验检测两组人舌鳞状细胞癌细胞CAL-27的侵袭性。通过明胶酶谱实验检测两组的基质金属蛋白酶2(matrix metalloproteinase,MMP-2)和MMP-9的活性。结果 IP-10组发生侵袭的细胞数为(1305±135)个/HP,对照组为(240±75)个/HP,差异有显著性(P<0.05)。IP-10组MMP-2和MMP-9的酶解量分别为(455.4±127.3)U和(492.1±161.5)U,对照组分别为(117.3±10.5)U和(166.7±39.1)U,差异均有显著性(P<0.05)。结论 IP-10可促进舌鳞状细胞癌细胞的侵袭作用。
        Objective To investigate the invasion influence of tongue squamous carcinoma cell CAL-27 induced by IP-10.Method The experiment was divided in two groups according to add the IP-10 or not,Ip-10(80 000 pg/ml) and the control group,using Transwell attack test to determine the impact of the CAL-27.The activity of MMP-2 and MMP-9 was detected through the gelatose spectrum experiment.Result The number of invasive cells in the IP-10 group was 1305±135,whereas the control group was 240±75,and the two groups had statistical differences(P<0.05).The amount of MMP-2 enzyme solution for the IP-10 group was(455.4±127.3) U,and the amount of MMP-9 enzyme solution for the IP-10 group was(492.1±161.5)U.In the control group,the amount of MMP-2 enzyme solution for the IP-10 group was(117.3±10.5) U,and the amount of MMP-9 enzyme solution for the IP-10 group was(166.7±39.1) U,and there was a statistical difference between the two groups(P<0.05).Conclusion IP-10 can promote the invasive function of the tongue squamous carcinoma cell.

引文

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