A亚型禽白血病的遗传抗性位点与肉鸡重要经济性状的关联分析
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摘要
为了研究A亚型禽白血病病毒遗传抗性与重要经济性状的关联性,本试验组建了肉鸡F_2代资源群体,在788只健康鸡中筛选了tva基因的抗性位点,分别测量记录每只鸡6、8、10、12周龄体重、屠宰体重、胸肌重、腿肌重等重要经济性状指标,并与tva基因位点进行关联分析。研究发现,在该试验群体中存在3个突变型tva~(r3)、tva~(r5)和tva~(r6),命名为tva~(mut502-516)。通过关联分析发现,tva~(mut502-516)位点在屠体性状方面,只与腹脂重(AFW)和腹脂率(AFR)呈显著相关(P<0.05),与生长、饲料转化率及其他屠宰性状的相关性均未达到显著水平(P>0.05)。研究结果表明,A亚型禽白血病病毒的抗性遗传突变可以显著降低肉鸡的腹脂重(AFW)和腹脂率(AFR),并且对肉鸡的生长、饲料转化率等其他重要经济性状没有产生不良的影响,因此,该抗性位点可以兼顾抗性和生长发育,可应用于A亚型禽白血病抗病育种。
To investigate the association of genetic resistance loci to subgroup A avian leukosis virus(ALV) with important economic traits, the genetic variations within resistance loci of tva gene were screened in an F_2 resource population of broilers, which contained 788 healthy chicken. Production-related traits such as body weight, breast muscle weight, leg muscle weight on 6, 8, 10, 12 weeks and slaughter weight were measured, separately, and their association with genetic resistance loci to subgroup A avian leukosis virus was analyzed. Three variants(tva~(r3), tva~(r5) and tva~(r6), named tva~(mut)~(502-516)) were discovered in the F_2 population. Association analysis revealed that the tva~(mut)~(502-516) was significantly associated with abdominal fat weight(AFW) and abdominal fat rate(AFR)(P<0.05), but not significantly associated with growth, feed efficiency and other carcass traits(P>0.05). These results indicate that the mutation of genetic resistance loci to subgroup A avian leukosis virus can significantly decrease the AFW and AFR of chicken, and had no unfavorable effect on growth, feed efficiency and other carcass traits, therefore, these loci can balance the ALV-A resistance and growth development, can be applied in subgroup A ALV resistant breeding of broilers.
To investigate the association of genetic resistance loci to subgroup A avian leukosis virus(ALV) with important economic traits, the genetic variations within resistance loci of tva gene were screened in an F_2 resource population of broilers, which contained 788 healthy chicken. Production-related traits such as body weight, breast muscle weight, leg muscle weight on 6, 8, 10, 12 weeks and slaughter weight were measured, separately, and their association with genetic resistance loci to subgroup A avian leukosis virus was analyzed. Three variants(tva~(r3), tva~(r5) and tva~(r6), named tva~(mut)~(502-516)) were discovered in the F_2 population. Association analysis revealed that the tva~(mut)~(502-516) was significantly associated with abdominal fat weight(AFW) and abdominal fat rate(AFR)(P<0.05), but not significantly associated with growth, feed efficiency and other carcass traits(P>0.05). These results indicate that the mutation of genetic resistance loci to subgroup A avian leukosis virus can significantly decrease the AFW and AFR of chicken, and had no unfavorable effect on growth, feed efficiency and other carcass traits, therefore, these loci can balance the ALV-A resistance and growth development, can be applied in subgroup A ALV resistant breeding of broilers.
引文
[1]PAYNE L N,NAIR V.The long view:40years of avian leukosis research[J].Avian Pathol,2012,41(1):11-19.
[2]LI X J,LIN W C,CHANG S,et al.Isolation,identification and evolution analysis of a novel subgroup of avian leukosis virus isolated from a local Chinese yellow broiler in South China[J].Arch Virol,2016,161(10):2717-2725.
[3]RAO M Z R,ZHAO Z J Z,YUAN L X Y,et al.The construction and application of a cell line resistant to novel subgroup avian leukosis virus(ALV-K)infection[J].Arch Virol,2018,163(1):89-98.
[4]KREAGER K S.Chicken industry strategies for control of tumor virus infections[J].Poult Sci,1998,77(8):1213-1216.
[5]LAI H Z,ZHANG H N,NING Z Y,et al.Isolation and characterization of emerging subgroup J avian leukosis virus associated with hemangioma in eggtype chickens[J].Vet Microbiol,2011,151(3-4):275-283.
[6]GAO Y L,YUN B L,QIN L T,et al.Molecular epidemiology of avian leukosis virus subgroup J in layer flocks in China[J].J Clin Microbiol,2012,50(3):953-960.
[7]FENG M,ZHANG X Q.Immunity to avian leukosis virus:Where are we now and what should we do?[J].Front Immunol,2016,7:624.
[8]PHOCAS F,BELLOC C,BIDANEL J,et al.Review:Towards the agroecological management of ruminants,pigs and poultry through the development of sustainable breeding programmes:I-selection goals and criteria[J].Animal,2016,10(11):1749-1759.
[9]BATES P,YOUNG J A T,VARMUS H E.A receptor for subgroup A Rous sarcoma virus is related to the low density lipoprotein receptor[J].Cell,1993,74(6):1043-1051.
[10]REINISOVAM,PLACHY J,TREJBALOVY′K,et al.Intronic deletions that disrupt mRNA splicing of thetvareceptor gene result in decreased susceptibility to infection by avian sarcoma and leukosis virus subgroup A[J].J Virol,2012,86(4):2021-2030.
[11]CHEN WG,LIU Y,LI H X,et al.Intronic deletions of tvareceptor gene decrease the susceptibility to infection by avian sarcoma and leukosis virus subgroup A[J].Sci Rep,2015,5:9900.
[12]NIE Q,SUN B,ZHANG D,et al.High diversity of the chicken growth hormone gene and effects on growth and carcass traits[J].J Hered,2005,96(6):698-703.
[13]PICKERING N K,DODDS K G,BLAIR H T,et al.Genetic parameters for production traits in New Zealand dual-purpose sheep,with an emphasis on dagginess[J].J Anim Sci,2012,90(5):1411-1420.
[14]EADY S J,WOOLASTON R R,PONZONI R W,et al.Resistance to nematode parasites in Merino sheep:Correlation with production traits[J].Aust J Agr Res,1998,49(8):1201-1211.
[15]HENRYON M,JOKUMSEN A,BERG P,et al.Erratum to“Genetic variation for growth rate,feed conversion efficiency,and disease resistance exists within a farmed population of rainbow trout”[Aquaculture209(2002)59-6][J].Aquaculture,2003,216(1-4):387-388.
[16]GITTERLE T,RYE M,SALTE R,et al.Genetic(co)variation in harvest body weight and survival in Penaeus(Litopenaeus)vannamei under standard commercial conditions[J].Aquaculture,2005,243(1-4):83-92.
[17]SHENG Z Y,PETTERSSON M E,HU X X,et al.Genetic dissection of growth traits in a Chinese indigenous×commercial broiler chicken cross[J].BMCGenomics,2013,14:151.
[18]SMITH J,GHEYAS A,BURT D W.Animal genomics and infectious disease resistance in poultry[J].Rev Sci Tech,2016,35(1):105-119.
[19]CHEN W G,LIU Y,LI H X,et al.Intronic deletions of tvareceptor gene decrease the susceptibility to infection by avian sarcoma and leukosis virus subgroup A[J].Sci Rep,2015,5:9900.
[20]KLUCKING S,ADKINS H B,YOUNG J A T.Resistance to infection by subgroups B,D,and E avian sarcoma and leukosis viruses is explained by apremature stop codon within a resistance allele of the tvbreceptor gene[J].J Virol,2002,76(15):7918-7921.
[21]REINISOVAM,SENIGL F,YIN X Q,et al.A single-amino-acid substitution in the TvbS1 receptor results in decreased susceptibility to infection by avian sarcoma and leukosis virus subgroups B and D and resistance to infection by subgroup Ein vitro and in vivo[J].J Virol,2008,82(5):2097-2105.
[22]LIU H C,KUNG H J,FULTON J E,et al.Growth hormone interacts with the Marek′s disease virus SORF2protein and is associated with disease resistance in chicken[J].Proc Natl Acad Sci U S A,2001,98(16):9203-9208.
[23]LIU H C,NIIKURA M,FULTON J E,et al.Identification of chicken lymphocyte antigen 6complex,locus E(LY6E,alias SCA2)as a putative Marek's disease resistance gene via a virus-host protein interaction screen[J].Cytogenet Genome Res,2003,102(1-4):304-308.
[24]DALGAARD T S,VITVED L,SKJDT K,et al.Molecular characterization of major histocompatibility complex class I(B-F)mRNA variants from chickens differing in resistance to marek's disease[J].Scand JImmunol,2005,62(3):259-270.
[25]GOTO R M,WANG Y J,TAYLOR R L Jr,et al.BG1has a major role in MHC-linked resistance to malignant lymphoma in the chicken[J].Proc Natl Acad Sci U S A,2009,106(39):16740-16745.
[26]LAMONT S J.Impact of genetics on disease resistance[J].Poult Sci,1998,77(8):1111-1118.
[27]SCANES C G.Perspectives on the endocrinology of poultry growth and metabolism[J].Gen Comp Endocrinol,2009,163(1-2):24-32.
[28]LI L,ZHANG X,KOVACIC S,et al.Identification of a human hollicular dendritic cell molecule that stimulates germinal benter B cell growth[J].J Exp Med,2000,191(6):1077-1084.
[29]YOUNG J A,BATES P,VARMUS H E.Isolation of a chicken gene that confers susceptibility to infection by subgroup A avian leukosis and sarcoma viruses[J].J Virol,1993,67(4):1811-1816.(编辑郭云雁)
[2]LI X J,LIN W C,CHANG S,et al.Isolation,identification and evolution analysis of a novel subgroup of avian leukosis virus isolated from a local Chinese yellow broiler in South China[J].Arch Virol,2016,161(10):2717-2725.
[3]RAO M Z R,ZHAO Z J Z,YUAN L X Y,et al.The construction and application of a cell line resistant to novel subgroup avian leukosis virus(ALV-K)infection[J].Arch Virol,2018,163(1):89-98.
[4]KREAGER K S.Chicken industry strategies for control of tumor virus infections[J].Poult Sci,1998,77(8):1213-1216.
[5]LAI H Z,ZHANG H N,NING Z Y,et al.Isolation and characterization of emerging subgroup J avian leukosis virus associated with hemangioma in eggtype chickens[J].Vet Microbiol,2011,151(3-4):275-283.
[6]GAO Y L,YUN B L,QIN L T,et al.Molecular epidemiology of avian leukosis virus subgroup J in layer flocks in China[J].J Clin Microbiol,2012,50(3):953-960.
[7]FENG M,ZHANG X Q.Immunity to avian leukosis virus:Where are we now and what should we do?[J].Front Immunol,2016,7:624.
[8]PHOCAS F,BELLOC C,BIDANEL J,et al.Review:Towards the agroecological management of ruminants,pigs and poultry through the development of sustainable breeding programmes:I-selection goals and criteria[J].Animal,2016,10(11):1749-1759.
[9]BATES P,YOUNG J A T,VARMUS H E.A receptor for subgroup A Rous sarcoma virus is related to the low density lipoprotein receptor[J].Cell,1993,74(6):1043-1051.
[10]REINISOVAM,PLACHY J,TREJBALOVY′K,et al.Intronic deletions that disrupt mRNA splicing of thetvareceptor gene result in decreased susceptibility to infection by avian sarcoma and leukosis virus subgroup A[J].J Virol,2012,86(4):2021-2030.
[11]CHEN WG,LIU Y,LI H X,et al.Intronic deletions of tvareceptor gene decrease the susceptibility to infection by avian sarcoma and leukosis virus subgroup A[J].Sci Rep,2015,5:9900.
[12]NIE Q,SUN B,ZHANG D,et al.High diversity of the chicken growth hormone gene and effects on growth and carcass traits[J].J Hered,2005,96(6):698-703.
[13]PICKERING N K,DODDS K G,BLAIR H T,et al.Genetic parameters for production traits in New Zealand dual-purpose sheep,with an emphasis on dagginess[J].J Anim Sci,2012,90(5):1411-1420.
[14]EADY S J,WOOLASTON R R,PONZONI R W,et al.Resistance to nematode parasites in Merino sheep:Correlation with production traits[J].Aust J Agr Res,1998,49(8):1201-1211.
[15]HENRYON M,JOKUMSEN A,BERG P,et al.Erratum to“Genetic variation for growth rate,feed conversion efficiency,and disease resistance exists within a farmed population of rainbow trout”[Aquaculture209(2002)59-6][J].Aquaculture,2003,216(1-4):387-388.
[16]GITTERLE T,RYE M,SALTE R,et al.Genetic(co)variation in harvest body weight and survival in Penaeus(Litopenaeus)vannamei under standard commercial conditions[J].Aquaculture,2005,243(1-4):83-92.
[17]SHENG Z Y,PETTERSSON M E,HU X X,et al.Genetic dissection of growth traits in a Chinese indigenous×commercial broiler chicken cross[J].BMCGenomics,2013,14:151.
[18]SMITH J,GHEYAS A,BURT D W.Animal genomics and infectious disease resistance in poultry[J].Rev Sci Tech,2016,35(1):105-119.
[19]CHEN W G,LIU Y,LI H X,et al.Intronic deletions of tvareceptor gene decrease the susceptibility to infection by avian sarcoma and leukosis virus subgroup A[J].Sci Rep,2015,5:9900.
[20]KLUCKING S,ADKINS H B,YOUNG J A T.Resistance to infection by subgroups B,D,and E avian sarcoma and leukosis viruses is explained by apremature stop codon within a resistance allele of the tvbreceptor gene[J].J Virol,2002,76(15):7918-7921.
[21]REINISOVAM,SENIGL F,YIN X Q,et al.A single-amino-acid substitution in the TvbS1 receptor results in decreased susceptibility to infection by avian sarcoma and leukosis virus subgroups B and D and resistance to infection by subgroup Ein vitro and in vivo[J].J Virol,2008,82(5):2097-2105.
[22]LIU H C,KUNG H J,FULTON J E,et al.Growth hormone interacts with the Marek′s disease virus SORF2protein and is associated with disease resistance in chicken[J].Proc Natl Acad Sci U S A,2001,98(16):9203-9208.
[23]LIU H C,NIIKURA M,FULTON J E,et al.Identification of chicken lymphocyte antigen 6complex,locus E(LY6E,alias SCA2)as a putative Marek's disease resistance gene via a virus-host protein interaction screen[J].Cytogenet Genome Res,2003,102(1-4):304-308.
[24]DALGAARD T S,VITVED L,SKJDT K,et al.Molecular characterization of major histocompatibility complex class I(B-F)mRNA variants from chickens differing in resistance to marek's disease[J].Scand JImmunol,2005,62(3):259-270.
[25]GOTO R M,WANG Y J,TAYLOR R L Jr,et al.BG1has a major role in MHC-linked resistance to malignant lymphoma in the chicken[J].Proc Natl Acad Sci U S A,2009,106(39):16740-16745.
[26]LAMONT S J.Impact of genetics on disease resistance[J].Poult Sci,1998,77(8):1111-1118.
[27]SCANES C G.Perspectives on the endocrinology of poultry growth and metabolism[J].Gen Comp Endocrinol,2009,163(1-2):24-32.
[28]LI L,ZHANG X,KOVACIC S,et al.Identification of a human hollicular dendritic cell molecule that stimulates germinal benter B cell growth[J].J Exp Med,2000,191(6):1077-1084.
[29]YOUNG J A,BATES P,VARMUS H E.Isolation of a chicken gene that confers susceptibility to infection by subgroup A avian leukosis and sarcoma viruses[J].J Virol,1993,67(4):1811-1816.(编辑郭云雁)