HPLC法同时测定肿痛安胶囊中5种成分的含量
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摘要
目的建立同时测定肿痛安胶囊中:天麻素、升麻素苷、5-O-甲基维斯阿米醇苷、欧前胡素和异欧前胡素含量的HPLC法。方法色谱柱:Phenomenex Luna C_(18)柱(250mm×4.6mm,5μm);流速:1.0mL·min~(-1);柱温:25℃;流动相:甲醇-水梯度洗脱;检测波长:230nm;进样量:5μL。结果天麻素质量浓度在12.46～199.30μg·mL~(-1)范围内线性关系良好,r_1=0.999 5,平均回收率为101.2%,RSD值为1.6%(n=6);升麻素苷质量浓度在12.07～193.20μg·mL~(-1)范围内线性关系良好,r_2=0.999 7,平均回收率为100.1%,RSD值为1.8%(n=6);5-O-甲基维斯阿米醇苷质量浓度在8.12～129.90μg·mL~(-1)范围内线性关系良好,r_3=0.999 8,平均回收率为99.1%,RSD值为1.7%(n=6);欧前胡素质量浓度在12.52～200.40μg·mL~(-1)范围内线性关系良好,r_4=0.999 6,平均回收率为98.6%,RSD值为1.4%(n=6);异欧前胡素质量浓度在12.65～202.40μg·mL~(-1)范围内线性关系良好,r_5=0.999 6,平均回收率为99.0%,RSD值为0.7%(n=6)。结论该方法简单、准确,可同时测定5种成分的含量,可用于肿痛安胶囊的质量控制。
Objective To establish an HPLC method for the determination of gastrodin,prim-O-glucosylcimifugin,5-O-methylvisammioside,imperatorin and isoimperatorin in Zhongtongan Capsules.Methods A Phenomenex Luna C_(18)column(250mm×4.6mm,5μm)was used and the column temperature was 25℃.The flow rate was 1.0mL·min~(-1)and the mobile phase consisted of methanol-water with gradient elution.The detection wavelength was 230nm and the injection volume was 5μL.Results The linear range of gastrodin was 12.46-199.30μg·mL~(-1)(r_1=0.999 5),and the average recovery was 101.2%(RSD=1.6%,n=6);the linear range of prim-O-glucosylcimifugin was 12.07-193.20μg·mL~(-1)(r_2=0.999 7),and the average recovery was 100.1%(RSD=1.8%,n=6);the linear range of 5-O-methylvisammioside was 8.12-129.90μg·mL~(-1)(r_3=0.999 8)and the average recovery was 99.1%(RSD=1.7%,n=6);the linear range of imperatorin was 12.52-200.40μg·mL~(-1)(r_4=0.999 6)and the average recovery was 98.6%(RSD=1.4%,n=6);the linear range of isoimperatorin was 12.65-202.40μg·mL~(-1)(r_5=0.999 6)and the average recovery was 99.0%(RSD=0.7%,n=6).Conclusion The method is simple,accurate and the content of 5ingredients can be determined simultaneously.The method can be used in the quality control of Zhongtongan Capsules.
Objective To establish an HPLC method for the determination of gastrodin,prim-O-glucosylcimifugin,5-O-methylvisammioside,imperatorin and isoimperatorin in Zhongtongan Capsules.Methods A Phenomenex Luna C_(18)column(250mm×4.6mm,5μm)was used and the column temperature was 25℃.The flow rate was 1.0mL·min~(-1)and the mobile phase consisted of methanol-water with gradient elution.The detection wavelength was 230nm and the injection volume was 5μL.Results The linear range of gastrodin was 12.46-199.30μg·mL~(-1)(r_1=0.999 5),and the average recovery was 101.2%(RSD=1.6%,n=6);the linear range of prim-O-glucosylcimifugin was 12.07-193.20μg·mL~(-1)(r_2=0.999 7),and the average recovery was 100.1%(RSD=1.8%,n=6);the linear range of 5-O-methylvisammioside was 8.12-129.90μg·mL~(-1)(r_3=0.999 8)and the average recovery was 99.1%(RSD=1.7%,n=6);the linear range of imperatorin was 12.52-200.40μg·mL~(-1)(r_4=0.999 6)and the average recovery was 98.6%(RSD=1.4%,n=6);the linear range of isoimperatorin was 12.65-202.40μg·mL~(-1)(r_5=0.999 6)and the average recovery was 99.0%(RSD=0.7%,n=6).Conclusion The method is simple,accurate and the content of 5ingredients can be determined simultaneously.The method can be used in the quality control of Zhongtongan Capsules.
引文
[1]WS3-B-3872-98-2005,国家食品药品监督管理局国家药品标准[S].
[2]国家药典委员会.中国药典:2015年版:一部[S].2015:58-59.
[3]周丽娟,申明慧,李敏,等.HPLC法测定蒺藜防白缓释滴丸中升麻素苷、5-O-甲基维斯阿米醇苷和欧前胡素[J].中成药,2015,37(12):2655-2660.
[4]陈建琴.UPLC法测定抗感解毒颗粒中欧前胡素、连翘苷、连翘酯苷A的含量[J].中国药师,2015,18(11):1876-1878.
[5]杨娟,林厚文,刘晓琰.HPLC法测定复方珍麻胶囊中天麻素的含量[J].中国药师,2016,19(2):408-409.
[6]余秀萍.RP-HPLC法测定玉真散胶囊中欧前胡素的含量[J].中国医院用药评价与分析,2016,16(1):275,277.
[7]薛朝金,文平,向丽霞.高效液相色谱法测定奢香天麻酒中天麻素的含量[J].北方药学,2018,15(5):4-5.
[8]田菁,李发美.HPLC测定感冒清热颗粒中升麻苷和5-O-甲基维斯阿米醇苷[J].中成药,2014,24(9):671-673.
[9]杨艳,郝丽晓,张秀丽.防风通圣丸中5-O-甲基维斯阿米醇苷含量测定方法的研究[J].山西中医学院学报,2014,15(3):39-41.
[10]郭琪,李向阳,宋汉敏.小儿健脾散中黄芪甲苷、欧前胡素和异欧前胡素的含量测定方法探索[J].中医研究,2014,27(9):71-75.
[11]姜燕.HPLC法同时测定玉真散中天麻素、升麻素苷、5-O-甲基维斯阿米醇苷、欧前胡素、异欧前胡素的含量[J].中国药师,2017,20(6):1147-1149.
[12]周丽娟,申明慧,李敏,等.HPLC法测定蒺藜防白缓释滴丸中升麻素苷、5-O-甲基维斯阿米醇苷和欧前胡素[J].中成药,2015,37(12):2655-2660.
[13]曹恒涛.高效液相色谱法测定黄连上清片中欧前胡素的含量[J].北方药学,2015,12(5):10-11.
[14]陈海燕,冷晓红,王秀芬,等.宁夏六盘山区栽培白芍的主要成分含量研究[J].西北药学杂志,2017,32(3):273-275.
[15]廖宝源,赖佐发,钟国庆,等.HPLC测定荆防止痒颗粒中升麻素苷和5-O-甲基维斯阿米醇苷的含量[J].赣南医学院学报,2017,37(1):55-57.
[16]姜雪敏,杨立志.高效液相色谱法测定感冒疏风片中升麻素苷及5-O-甲基维斯阿米醇苷含量[J].中国药业,2017,26(11):22-24.
[17]舒翔,叶小春,陈军,等.高效液相色谱法同时测定白芷中欧前胡素和异欧前胡素的含量[J].中国医院药学杂志,2017,37(5):427-429.
[18]唐德智.HPLC法同时测定藿香清胃胶囊中栀子苷、升麻素苷和5-O-甲基维斯阿米醇苷的含量[J].中药新药与临床药理,2016,27(5):701-704.
[19]谭润雅,雷鹏,周政,等.HPLC法同时测定枸橘中欧前胡素和异欧前胡素的含量[J].中药新药与临床药理,2016,27(2):255-258.
[2]国家药典委员会.中国药典:2015年版:一部[S].2015:58-59.
[3]周丽娟,申明慧,李敏,等.HPLC法测定蒺藜防白缓释滴丸中升麻素苷、5-O-甲基维斯阿米醇苷和欧前胡素[J].中成药,2015,37(12):2655-2660.
[4]陈建琴.UPLC法测定抗感解毒颗粒中欧前胡素、连翘苷、连翘酯苷A的含量[J].中国药师,2015,18(11):1876-1878.
[5]杨娟,林厚文,刘晓琰.HPLC法测定复方珍麻胶囊中天麻素的含量[J].中国药师,2016,19(2):408-409.
[6]余秀萍.RP-HPLC法测定玉真散胶囊中欧前胡素的含量[J].中国医院用药评价与分析,2016,16(1):275,277.
[7]薛朝金,文平,向丽霞.高效液相色谱法测定奢香天麻酒中天麻素的含量[J].北方药学,2018,15(5):4-5.
[8]田菁,李发美.HPLC测定感冒清热颗粒中升麻苷和5-O-甲基维斯阿米醇苷[J].中成药,2014,24(9):671-673.
[9]杨艳,郝丽晓,张秀丽.防风通圣丸中5-O-甲基维斯阿米醇苷含量测定方法的研究[J].山西中医学院学报,2014,15(3):39-41.
[10]郭琪,李向阳,宋汉敏.小儿健脾散中黄芪甲苷、欧前胡素和异欧前胡素的含量测定方法探索[J].中医研究,2014,27(9):71-75.
[11]姜燕.HPLC法同时测定玉真散中天麻素、升麻素苷、5-O-甲基维斯阿米醇苷、欧前胡素、异欧前胡素的含量[J].中国药师,2017,20(6):1147-1149.
[12]周丽娟,申明慧,李敏,等.HPLC法测定蒺藜防白缓释滴丸中升麻素苷、5-O-甲基维斯阿米醇苷和欧前胡素[J].中成药,2015,37(12):2655-2660.
[13]曹恒涛.高效液相色谱法测定黄连上清片中欧前胡素的含量[J].北方药学,2015,12(5):10-11.
[14]陈海燕,冷晓红,王秀芬,等.宁夏六盘山区栽培白芍的主要成分含量研究[J].西北药学杂志,2017,32(3):273-275.
[15]廖宝源,赖佐发,钟国庆,等.HPLC测定荆防止痒颗粒中升麻素苷和5-O-甲基维斯阿米醇苷的含量[J].赣南医学院学报,2017,37(1):55-57.
[16]姜雪敏,杨立志.高效液相色谱法测定感冒疏风片中升麻素苷及5-O-甲基维斯阿米醇苷含量[J].中国药业,2017,26(11):22-24.
[17]舒翔,叶小春,陈军,等.高效液相色谱法同时测定白芷中欧前胡素和异欧前胡素的含量[J].中国医院药学杂志,2017,37(5):427-429.
[18]唐德智.HPLC法同时测定藿香清胃胶囊中栀子苷、升麻素苷和5-O-甲基维斯阿米醇苷的含量[J].中药新药与临床药理,2016,27(5):701-704.
[19]谭润雅,雷鹏,周政,等.HPLC法同时测定枸橘中欧前胡素和异欧前胡素的含量[J].中药新药与临床药理,2016,27(2):255-258.